Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities | |
Li, Jiang1; Gu, Xiaoqian1; Pan, Aihong2 | |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019-04 | |
卷号 | 126页码:585-594 |
关键词 | Multifunctional enzyme alpha-Amylase Glycosyl hydrolase 70 |
ISSN号 | 0141-8130 |
DOI | 10.1016/j.ijbiomac.2018.12.217 |
英文摘要 | The open reading frame of an alpha-amylase coding gene, amy19, was obtained from a fosmid genomic library of hot spring bacterium Bacillus BI-19 by a plate-based assay of carrageenase activity. After heterologous expression of the gene, the recombinant Amy19 was found to possess alpha-amylase, agarase, carrageenase, and cellulase activities, and could degrade soluble starch, agarose, carrageen, and sodium cellulose into oligosaccharides with low degrees of polymerization. To explore the multifunctional mechanism of Amy19, three continuous glycosyl hydrolase 70 (GH70) motifs in the Amy19 encoding sequence were deleted one by one, then in pairs, then all at once. The GH70 motifs may play an important role in the multifunctionality of Amy19, but the multifunctionality was not determined by the GH70 motifs alone. To our knowledge, this is the first report of an alpha-amylase from a hot spring bacterium with additional agarase, carrageenase, and cellulase activities. (C) 2019 Elsevier B.V. All rights reserved. |
资助项目 | Public Science and Technology Research Funds Project of Ocean[201505026-4] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000460710000068 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/12773] ![]() |
专题 | 业务部门_海洋生态研究中心 |
通讯作者 | Li, Jiang |
作者单位 | 1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Jiang,Gu, Xiaoqian,Pan, Aihong. Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,126:585-594. |
APA | Li, Jiang,Gu, Xiaoqian,&Pan, Aihong.(2019).Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,126,585-594. |
MLA | Li, Jiang,et al."Multifunctional alpha-amylase Amy19 possesses agarase, carrageenase, and cellulase activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 126(2019):585-594. |
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