A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L

doi:10.1007/s10126-010-9309-8

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L
	Zhang, Pengying 2; Liu, Shenghao 1; Cong, Bailin 1; Wu, Guangting 1; Liu, Chenlin 1; Lin, Xuezheng 1; Shen, Jihong 1; Huang, Xiaohang 1
刊名	Marine Biotechnology
	2011-06-01
卷号	13 期号:3 页码:393-401
ISSN号	14362228
DOI	10.1007/s10126-010-9309-8
英文摘要	The ability of Antarctic ice algae, Chlamydomonas sp. ICE-L, to survive and proliferate at low temperature and high salinity implies that they have overcome key barriers inherent in Antarctic environments. A full-length complementary DNA (cDNA) sequence of omega-3 fatty acid desaturase, designated CiFAD3, was isolated via reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The full-length of CiFAD3 cDNA contained an open reading frame of 1,302 bp with 5′-terminal untranslated region (UTR) of 36 bp and 3′-terminal UTR of 507 bp encoding a fatty acid desaturase protein of 434 amino acids. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known chloroplastic omega-3 fatty acid desaturase. Meanwhile, CiFAD3 sequence showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane spanning regions that were universally present among plant desaturases. Under different stress conditions, messenger RNA (mRNA) expression levels of CiFAD3 were measured by quantitative RT-PCR. The results showed that both temperature and salinity could motivate the upregulation of CiFAD3 expression. The mRNA accumulation of CiFAD3 increased 2.6-fold at 0°C and 1.8-fold at 12°C compared to the algae at 6°C. Similarly, mRNA expression levels of CiFAD3 increased 3.8-fold after 62‰ NaCl treatment for 2 h. However, CiFAD3 mRNA expression levels were partially decreased after UV radiation. These data suggest that CiFAD3 is the enzyme responsible for the omega-3 fatty acid desaturation involved in ice algae Chlamydomonas sp. ICE-L acclimatizing to cold temperature and high salinity in Antarctic environment. © 2010 Springer Science+Business Media, LLC.
语种	英语
出版者	Springer New York LLC
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/6961]
专题	业务部门_海洋生态研究中心
作者单位	1.Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, Qingdao, Shandong 266061, China; 2.National Glycoengineering Research Center and College of Life Science, Shandong University, Jinan 250100, China
推荐引用方式 GB/T 7714	Zhang, Pengying,Liu, Shenghao,Cong, Bailin,et al. A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L[J]. Marine Biotechnology,2011,13(3):393-401.
APA	Zhang, Pengying.,Liu, Shenghao.,Cong, Bailin.,Wu, Guangting.,Liu, Chenlin.,...&Huang, Xiaohang.(2011).A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L.Marine Biotechnology,13(3),393-401.
MLA	Zhang, Pengying,et al."A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L".Marine Biotechnology 13.3(2011):393-401.