Cloning and expression of lipP, a gene encoding a cold-adapted lipase from Moritella sp.2-5-10-1

doi:10.1007/s00284-007-9051-2

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Cloning and expression of lipP, a gene encoding a cold-adapted lipase from Moritella sp.2-5-10-1
	Yang, Xiuxia 1; Lin, Xuezheng 2; Fan, Tingjun 1; Bian, Ji 2; Huang, Xiaohang 2
刊名	CURRENT MICROBIOLOGY
	2008-02
卷号	56 期号:2 页码:194-198
ISSN号	0343-8651
DOI	10.1007/s00284-007-9051-2
英文摘要	A gene (lipP, 837 bp in length) coding for a cold-adapted lipase of psychrophilic bacterium Moritella sp. 2-5-10-1 isolated from Antarctic region was cloned and sequenced in this study. The deduced amino acid sequence revealed a protein of 278 amino acid residues with a molecular mass of 30,521. The primary structure of the lipase deduced from the nucleotide sequence showed consensus pentapeptide containing the active serine [Gly-Trp-Ser-Leu-Gly] and a conserved His-Gly dipeptide in the N-terminal part of the enzyme. These sequences were involved in the lipase active site conformation. Structure factors that would allow proper enzyme flexibility at low temperatures were discussed. It was suggested that the changes in the primary structure of the psychrophilic lipases compared to the thermophilic ones could account for their ability to catalyze lipolysis at temperatures close to 0 degrees C. For expression, the sequence corresponding to the cold-adapted lipase of strain 2-5-10-1 was subcloned into the pET-28a expression vector to construct a recombinant lipase protein. Expression of the lipase by Escherichia coli BL21 (DE3) cells was observed as clear halos on 1% (vol/vol) tributyrin upon induction with IPTG at 25 degrees C.
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000252252600016
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4505]
专题	业务部门_海洋生态研究中心
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Dept Marine Biol, Qingdao 266003, Peoples R China; 2.Inst Oceanog 1, State Ocean Adm, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiuxia,Lin, Xuezheng,Fan, Tingjun,et al. Cloning and expression of lipP, a gene encoding a cold-adapted lipase from Moritella sp.2-5-10-1[J]. CURRENT MICROBIOLOGY,2008,56(2):194-198.
APA	Yang, Xiuxia,Lin, Xuezheng,Fan, Tingjun,Bian, Ji,&Huang, Xiaohang.(2008).Cloning and expression of lipP, a gene encoding a cold-adapted lipase from Moritella sp.2-5-10-1.CURRENT MICROBIOLOGY,56(2),194-198.
MLA	Yang, Xiuxia,et al."Cloning and expression of lipP, a gene encoding a cold-adapted lipase from Moritella sp.2-5-10-1".CURRENT MICROBIOLOGY 56.2(2008):194-198.