HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root | |
Han, Chao2; Shen, Yan3; Chen, Junhui1; Lee, Frank Sen-Chun1; Wang, Xiaoru1 | |
刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2008-02 | |
卷号 | 862期号:1-2页码:125-131 |
关键词 | fingerprint chromatogram Pseudostellaria heterophylla (Miq.) pax HPLC-TOF-MS hierarchical clustering analysis similarity analysis cyclic peptides |
ISSN号 | 1570-0232 |
DOI | 10.1016/j.jchromb.2007.11.041 |
英文摘要 | High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (LC-TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC-TOF-MS analysis; one cyclic peptide was unequivocally identified and five cyclic peptides were tentatively assigned based on their MS data. These cyclic peptides served as the marker peaks in the HPLC fingerprints. The chromatographic fingerprints have been analyzed by similarity index calculations and hierarchical clustering analysis (HCA). The result showed that the HPLC fingerprints could be used to determine the optimal harvest time for R heterophylla (Miq.) Pax and to authenticate the species of the herb. (c) 2007 Elsevier B.V. All rights reserved. |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000253354200014 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/4502] ![]() |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.First Inst Oceanog SOA, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China; 2.Wenzhou Entry Exit Inspect & Quarantine Bur, Wenzhou 325027, Peoples R China; 3.Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China |
推荐引用方式 GB/T 7714 | Han, Chao,Shen, Yan,Chen, Junhui,et al. HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,862(1-2):125-131. |
APA | Han, Chao,Shen, Yan,Chen, Junhui,Lee, Frank Sen-Chun,&Wang, Xiaoru.(2008).HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,862(1-2),125-131. |
MLA | Han, Chao,et al."HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 862.1-2(2008):125-131. |
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