On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl

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	On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl
	Wang Hong 1,2; Chen Jun-Hui 1; Zhao Heng-Qiang 1; Wang Lei-Lei 1,2; Zhang Dao-Lai 1; Wang Xiao-Ru 1,3,4; Yang Dong-Fang 2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2009-06
卷号	37 期号:6 页码:795-800
关键词	Tea antioxidants screening identification high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry
ISSN号	0253-3820
英文摘要	A new method based on the HPLC on-line scavenging diphenyl-1-picrylhydrazyl (DPPH*) radical activity and ESI-TOF/MS for the rapid screening and identification of radical scavengers from water extract of tea was developed. Water extract of tea was separated by HPLC. The outlet of HPLC flow cell was connected to a split valve in order to divert a flow to ESI-TOF/MS for on-line rapid identification of the antioxidants in water extract of tea, and another stream react post-column with the DPPH solution for on-line screening the antioxidants in water extract of tea. The results showed that eleven compounds in tea have scavenging radical activity, and all the eleven compounds including theanine, theogallin, gallocatechin grade, theophyline, tryptophan, epigallocatechin, epigallocatechin-3-gallate, table catechole, gallate, gallocatechin-3-gallate and catechin gallate were identified by ESI-TOF/MS according to literatures and data base. The method has also been used for several other natural products successfully. This method is reliable, efficient and it is a powerful tool for rapid screening and identification of free radical scavenging compounds in complex natural products.
WOS研究方向	Chemistry
语种	中文
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000267385100003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4397]
专题	业务部门_海洋生态研究中心
作者单位	1.First Inst Oceanog State Ocean Adm, QiagDao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China; 2.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 201303, Peoples R China; 3.Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Minist Educ, Xiamen 361005, Peoples R China; 4.Xiamen Univ, Key Lab Analyt Sci, Coll Chem & Chem Engn, Minist Educ, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Wang Hong,Chen Jun-Hui,Zhao Heng-Qiang,et al. On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(6):795-800.
APA	Wang Hong.,Chen Jun-Hui.,Zhao Heng-Qiang.,Wang Lei-Lei.,Zhang Dao-Lai.,...&Yang Dong-Fang.(2009).On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(6),795-800.
MLA	Wang Hong,et al."On-line Screening and Identification of Free Radical Scavenging Compounds in Water Extract of Tea by High Performance Liquid Chromatography-Mass Spectrometry-2,2-Diphenyl-1-Picrylhydrazyl".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.6(2009):795-800.