Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS | |
Shen, Yan2; Liu, Aili2; Ye, Mingde2; Wang, Li3; Chen, Junhui4; Wang, Xiao-ru4; Han, Chao1 | |
刊名 | CHROMATOGRAPHIA
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2009-08 | |
卷号 | 70期号:3-4页码:431-438 |
关键词 | Column liquid chromatography-mass spectrometry Microwave assisted extraction LC-DAD-ESI-MSn LC-ESI-TOF-MS Triterpene saponins Centella asiatica |
ISSN号 | 0009-5893 |
DOI | 10.1365/s10337-009-1152-6 |
英文摘要 | Centella asiatica (L.) Urban is a traditional herbal medicine used in Asiatic countries, and is commonly used to treat various wounds, leprosy, tuberculosis and lupus diseases. In this work, a new method based on microwave assisted extraction followed by liquid chromatography-diode array detection-electrospray ionization multistage tandem mass spectrometry analysis has been developed for the identification and quantification of biologically active constituents in C. asiatica, including asiatic acid, asiaticoside and madecassoside. The separation was performed on an Agilent Eclipse C-18 column (150 mm x 4.6 mm i.d., 5 mu m) with gradient elution of acetonitrile and 0.1% aqueous acetic acid within 50 min. Detection was performed at 205 nm. The calibration curves showed good linearity (r (2) > 0.9992). The limits of detection ranged from 1.2 to 1.6 mu g mL(-1). The intra- and inter-day precision was less than 3% and the recovery of the assay was in the range of 95.4-106.8%. The method was successfully applied to the quantification of the three constituents in different samples of C. asiatica. The results indicated that the developed method could be considered to be a simple, rapid and reliable method for the quality evaluation of C. asiatica. The samples were also analyzed on a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry system to confirm the identification results. |
资助项目 | Wenzhou Municipal Science and Technology Bureau[H20080052] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | SPRINGER HEIDELBERG |
WOS记录号 | WOS:000268995600011 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/4392] ![]() |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.Wenzhou Entry Exit Inspect & Quarantine Bur PRC, Wenzhou 325027, Peoples R China; 2.Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China; 3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol Engn, Hangzhou 310035, Zhejiang, Peoples R China; 4.SOA, Inst Oceanog 1, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Shen, Yan,Liu, Aili,Ye, Mingde,et al. Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS[J]. CHROMATOGRAPHIA,2009,70(3-4):431-438. |
APA | Shen, Yan.,Liu, Aili.,Ye, Mingde.,Wang, Li.,Chen, Junhui.,...&Han, Chao.(2009).Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS.CHROMATOGRAPHIA,70(3-4),431-438. |
MLA | Shen, Yan,et al."Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS".CHROMATOGRAPHIA 70.3-4(2009):431-438. |
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