Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO3 Microspheres and Gold Nanoparticles

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO3 Microspheres and Gold Nanoparticles
	Zheng Li 1; Jiang Feng-hua 1; Ma Guang-ran 2; Zhuang Qian-fen 2; Li Feng 2
刊名	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
	2011-09
卷号	27 期号:5 页码:875-879
关键词	Direct electrochemistry Horseradish peroxidase(HRP) CaCO3 microspherc Chitosan based sol-gel
ISSN号	1005-9040
英文摘要	Direct electrochemistry and electrocatalysis of horseradish peroxidase(HRP) were achieved by entrapping the enzyme between CaCO3 microspheres and gold nanoparticles through forming sandwich configuration (CaCO3-HRP-AuNPs). Polyanion, poly(styrene sulfonate)(PSS), was hybrid with CaCO3 microspheres to increase the surface negative charges for binding with HRP through electrostatic interaction. After the bioconjugate CaCO3 PSS-HRP was entrapped in chitosan based sol-gel(CS-GPTMS) film, HRP was encapsulated by in situ formation of an outer layer of AuNPs through electrochemical reduction of HAuCl4. The composite film containing AuNPs, Ca-CO3-PSS-HRP bioconjugates and CS-GPTMS can provide favorable microenvironment for HRP to perform direct electron transfer at glassy carbon electrode(GCE). HRP) retained its bioelectrocatalytic activity and lead to sensitive and fast amperometric response for the determination of H2O2. H2O2 could be detected in a very wide linear range from 5.0x10(-6) mol/L to 7.1x10(-2) mol/L. The sandwich configuration of CaCO3-biomolecules-AuNPs could serve as a versatile platform for enzyme immobilization and biosensing.
资助项目	Shandong Company of China National Tobacco Corporation[KN172]
WOS研究方向	Chemistry
语种	英语
出版者	HIGHER EDUCATION PRESS
WOS记录号	WOS:000296120800036
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4190]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Marine Ecol Res Ctr, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Shandong Prov Key Lab Biochem Anal, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Zheng Li,Jiang Feng-hua,Ma Guang-ran,et al. Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO3 Microspheres and Gold Nanoparticles[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(5):875-879.
APA	Zheng Li,Jiang Feng-hua,Ma Guang-ran,Zhuang Qian-fen,&Li Feng.(2011).Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO3 Microspheres and Gold Nanoparticles.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,27(5),875-879.
MLA	Zheng Li,et al."Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO3 Microspheres and Gold Nanoparticles".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 27.5(2011):875-879.