Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana

doi:10.1007/s00343-013-2168-1

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana
	Zheng Minggang 1; Liang Kepeng 2; Wang Bo 1; Sun Xiuqin 1; Yue Yanyan 3; Wan Wenwen 3; Zheng Li 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2013-03
卷号	31 期号:2 页码:398-406
关键词	Isochrysis galbana enoyl-ACP reductase real-time quantitative PCR temperature nitrogen
ISSN号	0254-4059
DOI	10.1007/s00343-013-2168-1
英文摘要	In most bacteria, plants and algae, fatty acid biosynthesis is catalyzed by a group of freely dissociable proteins known as the type II fatty acid synthase (FAS II) system. In the FAS II system, enoylacyl carrier protein reductase (ENR) acts as a determinant for completing the cycles of fatty acid elongation. In this study, the cDNA sequence of ENR, designated as IgENR, was isolated from the microalga Isochrysis galbana CCMM5001. RACE (rapid amplification of cDNA ends) was used to isolate the full-length cDNA of IgENR (1 503 bp), which contains an open reading frame (ORF) of 1 044 bp and encodes a protein of 347 amino acids. The genomic DNA sequence of IgENR is interrupted by four introns. The putative amino acid sequence is homologous to the ENRs of seed plants and algae, and they contain common coenzymebinding sites and active site motifs. Under different stress conditions, real-time quantitative polymerase chain reaction (RT-qPCR) showed the expression of IgENR was upregulated by high temperature (35A degrees C), and downregulated by depleted nitrogen (0 mol/L). To clarify the mechanism of lipids accumulating lipids, other genes involved in lipids accumulation should be studied.
资助项目	Science and Technology Development Program of Shandong Province[2011GHY11533]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000316018300016
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4002]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Inst Oceanog 1, Res Ctr Marine Ecol, Qingdao 266061, Peoples R China; 2.Shangdong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; 3.Shihezi Univ, Food Coll, Shihezi 832000, Peoples R China
推荐引用方式 GB/T 7714	Zheng Minggang,Liang Kepeng,Wang Bo,et al. Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):398-406.
APA	Zheng Minggang.,Liang Kepeng.,Wang Bo.,Sun Xiuqin.,Yue Yanyan.,...&Zheng Li.(2013).Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),398-406.
MLA	Zheng Minggang,et al."Isolation and characterization of an enoyl-acyl carrier protein reductase gene from microalga Isochrysis galbana".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):398-406.