Cloning and stress-respondent transcription of 3-ketoacyl-CoA thiolase gene of Isochrysis galbana (Haptophyta)

doi:10.1007/s10811-012-9904-3

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	Cloning and stress-respondent transcription of 3-ketoacyl-CoA thiolase gene of Isochrysis galbana (Haptophyta)
	Zheng, Minggang 1; Yue, Yanyan 2; Wan, Wenwen 2; Liang, Kepeng 3; Zheng, Li 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2013-04
卷号	25 期号:2 页码:695-702
关键词	3-Ketoacyl-CoA thiolase Gene transcription Isochrysis galbana Quantitative real-time PCR Sodium nitrate stress Temperature stress
ISSN号	0921-8971
DOI	10.1007/s10811-012-9904-3
英文摘要	Thiolases are crucial for both synthesis and catabolism of fatty acids, which catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA. The thiolase of a unicellular alga Isochrysis galbana (IgKAT) has not been identified yet. In this study, a full-length cDNA of 3-ketoacyl-CoA thiolase gene (IgKAT) was isolated with real-time PCR and rapid amplification of cDNA ends methods. IgKAT was 1,910 bp in length, containing an open reading frame of 1,188 bp, a 5'-terminal untranslated region (UTR) of 160 bp, and a 3'-UTR of 562 bp. The deduced amino acid sequence of IgKAT was 395 amino acids residues in length with a predicted molecular weight of 40.47 kDa and an isoelectric point of 6.53. IgKAT contained highly conserved domains and activity-center forming amino acids residues. In comparison with the normal temperature (25 A degrees C) and original content of sodium nitrate in f/2 medium (75 mg L-1), high temperature (35 A degrees C) and sodium nitrate limitation (0 mg L-1) upregulated the transcription of IgKAT gene, while low temperature (15 A degrees C) and sodium nitrate excess (150 mg L-1) downregulated the transcription of IgKAT gene in general. Our findings provided an understanding about the response of IgKAT to temperature and nutrition nitrate stresses (those deviating from the normal) at transcription level. These findings will aid to our understanding of the gene regulation of beta-oxidation pathway in response to culture conditions of I. galbana.
资助项目	Provincial Natural Science Foundation of Shandong[2010ZRA02013]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000316023400036
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3991]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm China, Res Ctr Marine Ecol, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Shihezi Univ, Shihezi 832000, Peoples R China; 3.Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Minggang,Yue, Yanyan,Wan, Wenwen,et al. Cloning and stress-respondent transcription of 3-ketoacyl-CoA thiolase gene of Isochrysis galbana (Haptophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(2):695-702.
APA	Zheng, Minggang,Yue, Yanyan,Wan, Wenwen,Liang, Kepeng,&Zheng, Li.(2013).Cloning and stress-respondent transcription of 3-ketoacyl-CoA thiolase gene of Isochrysis galbana (Haptophyta).JOURNAL OF APPLIED PHYCOLOGY,25(2),695-702.
MLA	Zheng, Minggang,et al."Cloning and stress-respondent transcription of 3-ketoacyl-CoA thiolase gene of Isochrysis galbana (Haptophyta)".JOURNAL OF APPLIED PHYCOLOGY 25.2(2013):695-702.