Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database | |
Li Zhao-Yong1; Chen Jun-Hui1; Li Xin1; Zhang Ru-Tan1; Chen Chen1; Shi Qian1; Wang Shuai1; Zheng Li1; Wang Xiao-Ru1,2 | |
刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY |
2013-07 | |
卷号 | 41期号:7页码:979-984 |
关键词 | Red tide Paralytic shellfish poisoning Hydrophilic interaction liquid chromatography High resolution mass spectrometry Screening |
ISSN号 | 0253-3820 |
DOI | 10.1016/S1872-2040(13)60665-9 |
英文摘要 | In this study, a new method was developed for the rapid screening and identification of paralytic shellfish poisoning (PSP) toxins in red tide algae by hydrophilic interaction liquid chromatography-high resolution mass spectrometry (HILIC-HR-MS) combined with accurate mass database. Toxic algae sample was extracted with 0.1 M acetic acid after cell-breaking, and then the crude extracts were analyzed by HILIC-HR-MS in positive and negative full scan modes. The accurate mass of quasi-molecular ion and main fragment ion peaks of PSP toxins in toxic algae were acquired and the PSP toxins in algae could be successfully qualitated by database screening and HR-MS data analysis using commercial software (Masshunter). The created database includes data not only the accurate relative molecular masses of 25 common PSP toxins but also the fragment ions. The results showed that the proposed HILIC-HR-ESI/MS method has high sensitive and separation efficiency. The limits of detection (LOD) of ten common PSP compounds were in the range of 10-80 nM, and it could meet the requirements for the actual screening of toxic algae samples. The developed method was applied to screen the PSP toxins in three kinds of toxic algae which demonstrated that the method was a useful tool for the rapid screening and qualitative identification of common PSP toxins in harmful algae. |
资助项目 | National Natural Scientific Foundation of China[20905017] |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE INC |
WOS记录号 | WOS:000324468800002 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3957] |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.State Ocean Adm, Res Ctr Marine Ecol, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China |
推荐引用方式 GB/T 7714 | Li Zhao-Yong,Chen Jun-Hui,Li Xin,et al. Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(7):979-984. |
APA | Li Zhao-Yong.,Chen Jun-Hui.,Li Xin.,Zhang Ru-Tan.,Chen Chen.,...&Wang Xiao-Ru.(2013).Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(7),979-984. |
MLA | Li Zhao-Yong,et al."Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.7(2013):979-984. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论