Rapid simultaneous determination of 15 nucleosides and nucleobases in marine medicinal organism Anthopleura lanthogrammica Berkly by micellar electrokinetic capillary chromatography

doi:10.1134/S1061934814020129

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Rapid simultaneous determination of 15 nucleosides and nucleobases in marine medicinal organism Anthopleura lanthogrammica Berkly by micellar electrokinetic capillary chromatography
	Shi, Q.1; Chen, J. H.1; Zhao, H. Q.1; Li, X.1; Zheng, L.1; Wang, X. R.1,2; Zang, J. Y.1
刊名	JOURNAL OF ANALYTICAL CHEMISTRY
	2014-02
卷号	69 期号:2 页码:169-178
关键词	marine medicinal organism Anthopleura lanthogrammica Berkly nucleosides nucleobases capillary electrophoresis
ISSN号	1061-9348
DOI	10.1134/S1061934814020129
英文摘要	In this study, a rapid and reliable micellar electrokinetic capillary chromatography (MECC) method has been developed and validated for the separation and quantification of 15 nucleosides and nucleobases in a kind of marine medicinal animal Anthopleura lanthogrammica Berkly. Factors such as micelle concentration, pH, concentration of buffer, etc. were investigated in order to obtain the optimum conditions for the separation. The optimized separation procedure employed 25 mM disodium hydrogen phosphate buffer containing 70 mM sodium dodecyl sulfate (SDS) at pH 8.7 as background electrolyte, 25A degrees C of capillary temperature and 20 kV of separation voltage. UV detection was at 260 nm and injection was hydrodynamic at 50 mbar for 3 s. The whole analysis was completed within 13 min. The relative standard deviations (RSDs) of the peak areas for method precision ranged from 1.4 to 7.6%, and the recovery was between 80.2 and 112.9%. The limit of detection (LOD) for 15 nucleosides and nuleobases was between 0.25 and 0.75 mu g/mL. According to the evaluation study, the method was shown to be precise, accurate and reproducible. The proposed MECC method was successfully applied for determination of nucleosides and nucleobases in Chinese Anthopleura lanthogrammica Berkly. The result showed that the species and content of nucleosides and nucleobases in Anthopleura lanthogrammica Berkly samples were abundant.
资助项目	Ministry of Science and Technology "863" project of China[2007AA092001-10]
WOS研究方向	Chemistry
语种	英语
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
WOS记录号	WOS:000330783600010
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3862]
专题	业务部门_海洋生态研究中心
作者单位	1.First Inst Oceanog, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China; 2.Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Shi, Q.,Chen, J. H.,Zhao, H. Q.,et al. Rapid simultaneous determination of 15 nucleosides and nucleobases in marine medicinal organism Anthopleura lanthogrammica Berkly by micellar electrokinetic capillary chromatography[J]. JOURNAL OF ANALYTICAL CHEMISTRY,2014,69(2):169-178.
APA	Shi, Q..,Chen, J. H..,Zhao, H. Q..,Li, X..,Zheng, L..,...&Zang, J. Y..(2014).Rapid simultaneous determination of 15 nucleosides and nucleobases in marine medicinal organism Anthopleura lanthogrammica Berkly by micellar electrokinetic capillary chromatography.JOURNAL OF ANALYTICAL CHEMISTRY,69(2),169-178.
MLA	Shi, Q.,et al."Rapid simultaneous determination of 15 nucleosides and nucleobases in marine medicinal organism Anthopleura lanthogrammica Berkly by micellar electrokinetic capillary chromatography".JOURNAL OF ANALYTICAL CHEMISTRY 69.2(2014):169-178.