Isolation and characterization of Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and fluoranthene by their syntrophic association with Marinobacter strains

doi:10.1016/j.ibiod.2014.03.005

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Isolation and characterization of Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and fluoranthene by their syntrophic association with Marinobacter strains
	Cui, Zhisong 1,2; Xu, Guangsu 2; Gao, Wei 2; Li, Qian 2; Yang, Baijuan 2; Yang, Guanpin 1; Zheng, Li 2
刊名	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
	2014-07
卷号	91 页码:45-51
关键词	High-molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) Cycloclasticus Yellow Sea Biodegradation Pyrene Fluoranthene
ISSN号	0964-8305
DOI	10.1016/j.ibiod.2014.03.005
英文摘要	Three Cycloclasticus strains, NY93E, PY97M, and PY97N, isolated from the sediments of Yellow Sea, were characterized. Interaction between Cycloclasticus strains and Marinobacter strains in the biodegradation of high-molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) was also studied. The three strains exhibited novel characteristics compared with their counterparts within the genus Cycloclasticus. When used as sole sources of carbon and energy, pyrene and fluoranthene can be degraded by each of the three strains, and the degradation rates of 0.02 g L-1 pyrene and fluoranthene determined by GC MS were in the range 52%-63% and 49%-65% after 21 d, respectively. The gene clusters encoding the iron sulfur protein of the PAH dioxygenase were PCR amplified from these strains and their nucleotide sequences exhibited more than 98% nucleotide similarity with their counterpart in Cycloclasticus sp. A5. In addition, the denaturing gradient gel electrophoresis (DGGE) profile revealed that Cycloclasticus spp. and Sphingomonas spp. were the dominant members in the phenanthrene-degrading consortium enriched from the Yellow Sea sediments. Furthermore, the defined consortium of Cycloclasticus sp. PY97M and Marinobacter sp. D15-8W exhibited obvious synergic effects in HMW PAH biodegradation. The degradation rates of 0.02 g L-1 pyrene and fluoranthene by the consortium increased from 63% 76% and 65% - 83% compared with that of the pure culture of strain PY97M, respectively. In conclusion, the three Cycloclasticus strains can degrade HMW PAHs pyrene and fluoranthene, and their syntrophic association with Marinobacter strains is promising in the bioremediation of HMW PAH-contaminated marine environment. (C) 2014 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[_501100001809] ; National Natural Science Foundation of China[40906062] ; National Natural Science Foundation of China[41076108]
WOS研究方向	Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000337658500007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3807]
专题	业务部门_海洋生态研究中心
作者单位	1.Ocean Univ China, Coll Marine Life, Qingdao 266063, Peoples R China; 2.State Ocean Adm China, First Inst Oceanog, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Cui, Zhisong,Xu, Guangsu,Gao, Wei,et al. Isolation and characterization of Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and fluoranthene by their syntrophic association with Marinobacter strains[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2014,91:45-51.
APA	Cui, Zhisong.,Xu, Guangsu.,Gao, Wei.,Li, Qian.,Yang, Baijuan.,...&Zheng, Li.(2014).Isolation and characterization of Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and fluoranthene by their syntrophic association with Marinobacter strains.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,91,45-51.
MLA	Cui, Zhisong,et al."Isolation and characterization of Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and fluoranthene by their syntrophic association with Marinobacter strains".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 91(2014):45-51.