One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry

doi:10.1016/j.chroma.2014.06.023

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	One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry
	Lin, Zian 1; Tan, Xiaoqing 1; Yu, Ruifang 1; Lin, Jiashi 2; Yin, Xiaofei 3; Zhang, Lan 1; Yang, Huanghao 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2014-08-15
卷号	1355 页码:228-237
关键词	Capillary liquid chromatography Organic-inorganic hybrid monolith Glutathione Click chemistry One-pot Mixed-mode
ISSN号	0021-9673
DOI	10.1016/j.chroma.2014.06.023
英文摘要	A novel glutathione (GSH)-silica hybrid monolithic column synthesized via a combination of thiolene click reaction and one-pot process was described, where thiol-end GSH organic monomer and 2,2-azobisisobutyronitrile (AIBN) were mixed with hydrolyzed tetramethyloxysilane (TMOS) and gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS) and then introduced into a fused-silica capillary for simultaneous polycondensation and "thiol-ene" click reaction to form the GSH-silica hybrid monolith. The effects of the molar ratio of TMOS/gamma-MAPS, the amount of GSH, and the volume of porogen on the morphology, permeability and pore properties of the prepared GSH-silica hybrid monoliths were studied in detail. A uniform monolithic network with high porosity was obtained. A series of test compounds including alkylbenzenes, amides, and anilines were used to evaluate the retention behaviors of the GSH-silica hybrid monolithic column. The results demonstrated that the prepared GSH-silica hybrid monolith exhibited multiple interactions including hydrophobicity, hydrophilicity, as well as cation exchange interaction. The run-to-run, column-to-column and batch-to-batch reproducibilities of the GSH-silica hybrid monolith for phenols' retention were satisfactory with the relative standard deviations (RSDs) less than 1.3% (n=5), 2.6% (n =3) and 3.2% (n=3), respectively, indicating the effectiveness and practicability of the proposed method. In addition, the GSH-silica hybrid monolith was applied to the separation of nucleotides, peptides and protein tryptic digests, respectively. The successful applications suggested the potential of the GSH-silica hybrid monolith in complex sample analysis. (C) 2014 Elsevier B.V. All rights reserved.
电子版国际标准刊号	18733778
资助项目	Program for Changjiang Scholars and Innovative Research Team in University[IRT1116]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000340302500025
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3793]
专题	业务部门_海洋生态研究中心
作者单位	1.Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China; 2.Jimei Univ, Coll Phys Educ, Xiamen 361021, Peoples R China; 3.SOA, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Lin, Zian,Tan, Xiaoqing,Yu, Ruifang,et al. One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1355:228-237.
APA	Lin, Zian.,Tan, Xiaoqing.,Yu, Ruifang.,Lin, Jiashi.,Yin, Xiaofei.,...&Yang, Huanghao.(2014).One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry.JOURNAL OF CHROMATOGRAPHY A,1355,228-237.
MLA	Lin, Zian,et al."One-pot preparation of glutathione-silica hybrid monolith for mixed-mode capillary liquid chromatography based on "thiol-ene" click chemistry".JOURNAL OF CHROMATOGRAPHY A 1355(2014):228-237.