Effect of nitrogen and temperature on the transcription of an ACAT gene in Isochrysis galbana

doi:10.1007/s11033-014-3608-5

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Effect of nitrogen and temperature on the transcription of an ACAT gene in Isochrysis galbana
	Huang, Yijiang 1; Zheng, Minggang 2; Wan, Wenwen 3; Sun, Zhongtao 1
刊名	MOLECULAR BIOLOGY REPORTS
	2014-11
卷号	41 期号:11 页码:7235-7240
关键词	Acetyl CoA acetyltransferase Isochrysis galbana Quantitative real-time PCR Stress-respondent transcription Triterpene
ISSN号	0301-4851
DOI	10.1007/s11033-014-3608-5
英文摘要	Thiolases are functionally divided into two groups: 3-ketoacyl-CoA thiolase and acetoacetyl-CoA thiolase (ACAT). Acetoacetyl-CoA thiolase plays a key role in the mevalonate pathway. In this study, a novel gene, IgACAT, which encodes ACAT was cloned from Isochrysis galbana and characterized. The cDNA of IgACAT was 1551 bp in length, consisting of an open reading frame of 1173 bp, a 50 untranslated region of 69 bp and a 30 untranslated region of 309 bp. The deduced amino acid sequence of IgACAT was 390 amino acid residues in length with a predicted molecular weight of 53.59 kDa and an isoelectric point of pH 9.04. The triterpenes content and the expression of IgACAT under nitrogen and temperature stress were analyzed. When I. galbana was treated with excessive nitrogen and at 35 degrees C, respectively, both the triterpenes content and the abundance of IgACAT gene transcript increased. Our findings will facilitate the regulation of gene expression and genetic modification of the triterpenes synthesis pathway of I. galbana.
资助项目	Ocean Industry Research Special Project[200905019]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000344101300023
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3759]
专题	业务部门_海洋生态研究中心
作者单位	1.Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China; 2.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China; 3.Shihezi Univ, Shihezi 832000, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yijiang,Zheng, Minggang,Wan, Wenwen,et al. Effect of nitrogen and temperature on the transcription of an ACAT gene in Isochrysis galbana[J]. MOLECULAR BIOLOGY REPORTS,2014,41(11):7235-7240.
APA	Huang, Yijiang,Zheng, Minggang,Wan, Wenwen,&Sun, Zhongtao.(2014).Effect of nitrogen and temperature on the transcription of an ACAT gene in Isochrysis galbana.MOLECULAR BIOLOGY REPORTS,41(11),7235-7240.
MLA	Huang, Yijiang,et al."Effect of nitrogen and temperature on the transcription of an ACAT gene in Isochrysis galbana".MOLECULAR BIOLOGY REPORTS 41.11(2014):7235-7240.