Proteomic Approaches of Trichoderma hamatum to Control Ralstonia solanacearum Causing Pepper Bacterial Wilt | |
Cheng, Peng1,2; Song, Wei4; Gong, Xiao1,2; Liu, Yisong3; Xie, Weiguo1,2; Huang, Lihua1,2; Hong, Yahui1,2 | |
刊名 | INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
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2015 | |
卷号 | 17期号:6页码:1101-1109 |
关键词 | Trichoderma hamatum Antimicrobial Activity Bacterial wilt Pepper Isolation and identification |
ISSN号 | 1560-8530 |
DOI | 10.17957/IJAB/15.0049 |
英文摘要 | Hunan province has the largest pepper cultivation and highest pepper consumption in China In recent years, peppers (Capsicum frutescens L.) cultivated in Hunan province have been infected by Ralstonia solanacearum, causing bacterial wilt thus inducing vast economic losses. Twenty-two strains of Trichoderma were isolated from soil samples near the pepper cultivation area in LiLing, Hunan province, PR China Phylogenetic analysis based on the ITS region of the isolated strains detected three species including Trichoderma hamatum, T. asperellum, and T. virens. An antimicrobial test showed that T. hamatum strain YYH13 had the greatest antimicrobial activity against the bacterial wilt pathogen and that proteins in the liquid metabolites played a major role in this activity. A 1D-shotgun proteomics approach, in conjunction with LC-MS/MS, identified 125 different secreted proteins. Most of the antimicrobial proteins contain several specific chitinases that play an important role as hydrolytic enzymes during cell wall degradation. (C) 2015 Friends Science Publishers |
资助项目 | College students innovative experiment plan of Hunan province[SCX1419] |
WOS研究方向 | Agriculture ; Life Sciences & Biomedicine - Other Topics |
语种 | 英语 |
出版者 | FRIENDS SCIENCE PUBL |
WOS记录号 | WOS:000366737400004 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3734] ![]() |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | 1.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China; 2.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China; 3.Hunan Agr Univ, Natl Chinese Med Herbs Hunan Technol Ctr, Changsha 410128, Hunan, Peoples R China; 4.SOA, Inst Oceanog 1, Key Lab Sci & Engn Marine Ecol & Environm, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Cheng, Peng,Song, Wei,Gong, Xiao,et al. Proteomic Approaches of Trichoderma hamatum to Control Ralstonia solanacearum Causing Pepper Bacterial Wilt[J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,2015,17(6):1101-1109. |
APA | Cheng, Peng.,Song, Wei.,Gong, Xiao.,Liu, Yisong.,Xie, Weiguo.,...&Hong, Yahui.(2015).Proteomic Approaches of Trichoderma hamatum to Control Ralstonia solanacearum Causing Pepper Bacterial Wilt.INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,17(6),1101-1109. |
MLA | Cheng, Peng,et al."Proteomic Approaches of Trichoderma hamatum to Control Ralstonia solanacearum Causing Pepper Bacterial Wilt".INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY 17.6(2015):1101-1109. |
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