Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide

doi:10.1007/s13131-016-0892-z

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide
	Lin Xuezheng; Wang Zhen; Li Yang; Li Jiang
刊名	ACTA OCEANOLOGICA SINICA
	2016-12
卷号	35 期号:12 页码:73-80
关键词	Arctic Pseudoalteromonas oxidative stress RNA-Seq
ISSN号	0253-505X
DOI	10.1007/s13131-016-0892-z
英文摘要	Oxidative stress is one of the major challenges faced by Arctic marine bacteria due to the high oxygen concentration of seawater, low temperatures and UV radiations. Transcriptome sequencing was performed to obtain the key functional genes involved in the adaptation to oxidative stress induced by hydrogen peroxide in the Arctic bacterium Pseudoalteromonas sp. A2. Exposure to 1 mmol/L H2O2 resulted in large alterations of the transcriptome profile, including significant up-regulation of 109 genes and significant down-regulation of 174 genes. COG functional classification revealed that among the significantly regulated genes with known function categories, more genes belonging to posttranslational modification, protein turnover and chaperones were significantly up-regulated, and more genes affiliated with chaperones and amino acid transport and metabolism were significantly down-regulated. It was notable that the expressions of eighteen genes affiliated with flagella and four genes affiliated with heat shock proteins were significantly up-regulated. Meanwhile, the expression of nine genes belonging to cytochrome and cytochrome oxidase, and five genes belonging to TonB-dependent receptor, were significantly down-regulated. Among the eighteen genes with antioxidant activity categorized by GO analysis, the expression of one gene was significantly up-regulated; however, the expressions of two genes were significantly down-regulated. Briefly, RNA-Seq indicated that, except for the classical anti-oxidative genes and stress proteins, genes affiliated with flagella and function unknown played important roles in coping with oxidative stress in Pseudoalteromonas sp. A2. This overall survey of transcriptome and oxidative stress-relevant genes can contribute to understand the adaptive mechanism of Arctic bacteria.
资助项目	Public Science and Technology Funds for Ocean Projects[201205020-5]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000391423600009
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3298]
专题	业务部门_海洋生态研究中心
作者单位	State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Lin Xuezheng,Wang Zhen,Li Yang,et al. Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide[J]. ACTA OCEANOLOGICA SINICA,2016,35(12):73-80.
APA	Lin Xuezheng,Wang Zhen,Li Yang,&Li Jiang.(2016).Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide.ACTA OCEANOLOGICA SINICA,35(12),73-80.
MLA	Lin Xuezheng,et al."Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide".ACTA OCEANOLOGICA SINICA 35.12(2016):73-80.