Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide | |
Lin Xuezheng; Wang Zhen; Li Yang; Li Jiang | |
刊名 | ACTA OCEANOLOGICA SINICA
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2016-12 | |
卷号 | 35期号:12页码:73-80 |
关键词 | Arctic Pseudoalteromonas oxidative stress RNA-Seq |
ISSN号 | 0253-505X |
DOI | 10.1007/s13131-016-0892-z |
英文摘要 | Oxidative stress is one of the major challenges faced by Arctic marine bacteria due to the high oxygen concentration of seawater, low temperatures and UV radiations. Transcriptome sequencing was performed to obtain the key functional genes involved in the adaptation to oxidative stress induced by hydrogen peroxide in the Arctic bacterium Pseudoalteromonas sp. A2. Exposure to 1 mmol/L H2O2 resulted in large alterations of the transcriptome profile, including significant up-regulation of 109 genes and significant down-regulation of 174 genes. COG functional classification revealed that among the significantly regulated genes with known function categories, more genes belonging to posttranslational modification, protein turnover and chaperones were significantly up-regulated, and more genes affiliated with chaperones and amino acid transport and metabolism were significantly down-regulated. It was notable that the expressions of eighteen genes affiliated with flagella and four genes affiliated with heat shock proteins were significantly up-regulated. Meanwhile, the expression of nine genes belonging to cytochrome and cytochrome oxidase, and five genes belonging to TonB-dependent receptor, were significantly down-regulated. Among the eighteen genes with antioxidant activity categorized by GO analysis, the expression of one gene was significantly up-regulated; however, the expressions of two genes were significantly down-regulated. Briefly, RNA-Seq indicated that, except for the classical anti-oxidative genes and stress proteins, genes affiliated with flagella and function unknown played important roles in coping with oxidative stress in Pseudoalteromonas sp. A2. This overall survey of transcriptome and oxidative stress-relevant genes can contribute to understand the adaptive mechanism of Arctic bacteria. |
资助项目 | Public Science and Technology Funds for Ocean Projects[201205020-5] |
WOS研究方向 | Oceanography |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000391423600009 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/3298] ![]() |
专题 | 业务部门_海洋生态研究中心 |
作者单位 | State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Lin Xuezheng,Wang Zhen,Li Yang,et al. Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide[J]. ACTA OCEANOLOGICA SINICA,2016,35(12):73-80. |
APA | Lin Xuezheng,Wang Zhen,Li Yang,&Li Jiang.(2016).Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide.ACTA OCEANOLOGICA SINICA,35(12),73-80. |
MLA | Lin Xuezheng,et al."Genome-wide transcriptional response of the Arctic bacterium Pseudoalteromonas sp A2 to oxidative stress induced by hydrogen peroxide".ACTA OCEANOLOGICA SINICA 35.12(2016):73-80. |
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