Transcriptome profiling of the hypothalamus and pituitary at gonadal maturation and regression phases in marbled flounder (Pseudopleuronectes yokohamae)

doi:10.1007/s13258-016-0479-2

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Transcriptome profiling of the hypothalamus and pituitary at gonadal maturation and regression phases in marbled flounder (Pseudopleuronectes yokohamae)
	Zheng, Fengrong 1; Liu, Hongzhan 2; Shen, Zhen 2; Li, Qing 2; Zhang, Yongqiang 1; Wang, Bo 1
刊名	GENES & GENOMICS
	2017-07
卷号	39 期号:7 页码:713-722
关键词	Pseudopleuronectes yokohamae Transcriptome qRT-PCR Reproductive endocrine
ISSN号	1976-9571
DOI	10.1007/s13258-016-0479-2
英文摘要	The Pseudopleuronectes yokohamae (marbled flounder) features one of the most commercially important marine fishes in P.R. China. Previous studies were mainly focused on the biology and genetic breeding of the fish, little have been reported about the molecular mechanism of its reproduction. In this study, we used second generation Illumina sequencing to investigate the genes of Pseudopleuronectes yokohamae that regulate gonadal development and obtained 40,882 unigenes from the hypothalamus and pituitary samples of the fish at maturation and regression phases. Homogonous sequences of 29,129 unigenes (71.25 %) were identified and 308 unigenes among which were associated with the regulation of reproductive endocrine. These genes are a very useful resource for future studies of P.yokohamae. All unigenes with eggNog annotations were divided into 26 categories that are mostly involved in signal transduction, translation mechanism, physiological and biochemical processes, etc. Based on KEGG analysis, all unigenes expressed at the maturation phase were involved in 107 metabolic pathways, with 184 up-regulated sequences, while the unigenes expressed at the regression phase were related to 210 metabolic pathways, with 858 up-regulated sequences. Additionally, the quantitative real-time PCR results indicated that follicle stimulating hormone and prolactin-releasing peptide receptor are highly expression at the regression phase, while growth hormone releasing hormone, kisspeptin, gonadotropin and insulin growth-like factors expressed higher at the maturation phase. This study provides valuable data for better understanding the transcriptome profiles of marbled flounder and may help to improve artificial reproduction technologies of the fish.
资助项目	National High Technology Research and Development Program of China (863 Program)[2012AA10A413]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000404169800003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3133]
专题	业务部门_海洋生态研究中心
作者单位	1.SOA, Inst Oceanog 1, Ecol Lab, 6 Xianxialing Rd, Qingdao 266061, Shandong, Peoples R China; 2.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fengrong,Liu, Hongzhan,Shen, Zhen,et al. Transcriptome profiling of the hypothalamus and pituitary at gonadal maturation and regression phases in marbled flounder (Pseudopleuronectes yokohamae)[J]. GENES & GENOMICS,2017,39(7):713-722.
APA	Zheng, Fengrong,Liu, Hongzhan,Shen, Zhen,Li, Qing,Zhang, Yongqiang,&Wang, Bo.(2017).Transcriptome profiling of the hypothalamus and pituitary at gonadal maturation and regression phases in marbled flounder (Pseudopleuronectes yokohamae).GENES & GENOMICS,39(7),713-722.
MLA	Zheng, Fengrong,et al."Transcriptome profiling of the hypothalamus and pituitary at gonadal maturation and regression phases in marbled flounder (Pseudopleuronectes yokohamae)".GENES & GENOMICS 39.7(2017):713-722.