Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus)

doi:10.1111/are.13237

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus)
	Zheng, Fengrong 1; Liu, Hongzhan 2; Sun, Xiuqin 1; Qin, Xiaoping 1; Xu, Zongjun 1; Wang, Bo 1
刊名	AQUACULTURE RESEARCH
	2017-08
卷号	48 期号:8 页码:4174-4183
关键词	eukaryotic expression vector construction turbot reddish body iridovirus immune response immune efficacy DNA vaccine
ISSN号	1355-557X
DOI	10.1111/are.13237
英文摘要	Turbot aquaculture is a very important industry in China. However, it is hampered because of viral reddish body syndrome (VRBS) and high mortality caused by piscine turbot reddish body iridovirus (TRBIV). TRBIV virus is an icosahedron-like and cytoplasmic DNA virus, belonging to Iridoviridae, Megalocytivirus. In previous studies, we have identified two antigen mimotopes using bioinformatics and constructed prokaryotic expression vectors. In this study, a fragment of major capsid protein (MCP) gene with the two antigenic epitopes was cloned into eukaryotic expression vector pVAX1, to generate a recombinant plasmid pVAX1-TRBIV-MCP. The plasmid DNA was transferred into turbot cell line TK using liposome, and transient expression was detected using RT-PCR. After injection into turbot (Scophthalmus maximus), the expression of the antigen gene was analysed using RT-PCR and was shown to express in all tested tissues in vaccinated fish 2 and 7days post-vaccination. The cumulative mortalities in the vaccinated and unvaccinated control fish were 30% and 88% respectively. Immune responses and upregulation of the expression of chemokine receptor, tumour necrosis factor, interferon and interferon-induced antiviral molecules were observed in the vaccinated fish 60h post-vaccination. These results demonstrate that the vaccinated turbots had higher survival rate and produced specific serum antibodies following the TRBIV challenge. More studies are needed to develop and apply the promising DNA vaccine for virus control in turbot.
资助项目	National Marine Public Welfare Research Project[201305005] ; National Marine Public Welfare Research Project[201405010]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000404982300016
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3110]
专题	业务部门_海洋生态研究中心
作者单位	1.First Inst Oceanog SOA, Qingdao, Peoples R China; 2.Shandong Univ, Marine Coll, Weihai, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fengrong,Liu, Hongzhan,Sun, Xiuqin,et al. Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus)[J]. AQUACULTURE RESEARCH,2017,48(8):4174-4183.
APA	Zheng, Fengrong,Liu, Hongzhan,Sun, Xiuqin,Qin, Xiaoping,Xu, Zongjun,&Wang, Bo.(2017).Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus).AQUACULTURE RESEARCH,48(8),4174-4183.
MLA	Zheng, Fengrong,et al."Construction and expression of DNA vaccine against reddish body iridovirus and evaluation of immune efficacy in turbot (Scophthalmus maximus)".AQUACULTURE RESEARCH 48.8(2017):4174-4183.