Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp NJ-21

doi:10.1007/s00343-018-7074-0

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp NJ-21
	Xie Maisheng 1; Li Jiang 1,2; He Peiqing 1,2; Lin Xuezheng 1,2
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2018-07
卷号	36 期号:4 页码:1304-1314
关键词	Pseudoalteromonas sp. alginate lyase PL-6 family
ISSN号	2096-5508
DOI	10.1007/s00343-018-7074-0
英文摘要	In this study, an endolytic alginate lyase, named Al163, was identified, cloned, and characterized from the Antarctic bacterium Pseudoalteromonas sp. NJ-21. Comparative sequence analysis showed that the predicted amino acid sequence encoded by al163 belongs to the polysaccharide lyase 6 (PL-6) family and has a molecular mass of about 80 kDa. Recombinant enzyme was purified by Ni-Sepharose affinity chromatography. Recombinant Al163 exhibited maximum activity (258 U/mg) at pH 7.0 and 40 degrees C, and thermal stability assays showed retention of almost 90% activity after incubation at 30 degrees C for 30 min. Al163 activity was stimulated by Cd2+, Ca2+, Fe (3+), and Mn2+, but inhibited by Cu2+, Si2+, Fe2+, and Ni2+. Thin-layer chromatographic analysis indicated that Al163 degraded sodium alginate, polyM, and polyG, generating disaccharides and trisaccharides as the final products. Only a few bacterial strains that produce a bifunctional alginate lyase have been reported. Our results indicate that recombinant Al163 exhibits broad substrate specificity and its products exhibit low degrees of polymerization. Both properties imply high potential for use of the enzyme in several industrial fields, including cosmetics and pharmaceuticals, based on the high demand for biologically active oligosaccharides.
资助项目	Qingdao Applied Basic Research Project[14-2-4-14-jch]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000441231100025
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/2858]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm SOA, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China; 2.Key Lab Marine Nat Prod Qingdao, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Xie Maisheng,Li Jiang,He Peiqing,et al. Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp NJ-21[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1304-1314.
APA	Xie Maisheng,Li Jiang,He Peiqing,&Lin Xuezheng.(2018).Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp NJ-21.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1304-1314.
MLA	Xie Maisheng,et al."Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp NJ-21".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1304-1314.