Selection, identification and application of antagonistic bacteria associated with skin ulceration and peristome tumescence of cultured sea cucumber Apostichopus japonicus (Selenka)

doi:10.1016/j.aquaculture.2011.11.009

	Selection, identification and application of antagonistic bacteria associated with skin ulceration and peristome tumescence of cultured sea cucumber Apostichopus japonicus (Selenka)
	Zheng, Fengrong 1; Liu, Hongzhan 2; Sun, Xiuqin 1; Qu, Lingyun ; Dong, Shuanglin 3; Liu, Junling
刊名	AQUACULTURE
	2012-03-07
卷号	334 页码:24-29
关键词	Antagonistic bacteria Apostichopus japonicus Skin ulceration Peristome tumescence
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2011.11.009
英文摘要	The aquaculture of the sea cucumber Apostichopus japonicus has grown rapidly over recent years in China and become an important sector of the marine industry. However, with rapid growth and the use of non-standard practices and culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. Prevention and control of diseases seriously affecting aquaculture of A. japonicus, a major marine industry in China, are essential for its development. We have previously isolated two pathogenic bacteria Pseudoalteromonas sp and Pseudoalteromonas tetraodonis associated with ulceration and peristome tumescence of A. japonicus. In this study, we isolated 266 bacteria from A. japonicus and its cultural environment, and identified 5 bacteria Vibrio ordalii, Bacillus safensis, P. tetraodonis, Pseudoalteromonas elyakovii and Pseudomonas segetis with strong antimicrobial activity to P. sp and P. tetraodonis using point inoculation, filter paper and Oxford cylinder methods. Further investigation on their growth and antagonistic characteristics indicated that 1) V. ordalii has the strongest antimicrobial ability even at low temperature and its extracellular products precipitated with ammonium sulfate at different growth stages also have antimicrobial activity; 2) P. tetraodonis and P. elyakovii only have antimicrobial activity at 28 degrees C and their extracellular products at different growth stages also have strong antagonistic effects; 3) the extracellular products of B. safensis at different growth stages have no antibacterial activity. Injection of extracellular products of P. elyakovii and V. ordalii into A. japonicus can enhance its non-specific immune-related enzyme activities and disease resistance. The study provides a theoretical basis and products for A. japonicus disease prevention and healthy aquaculture. (C) 2011 Published by Elsevier B.V.
资助项目	Key Project of Youth Marine Science[2009119]
WOS关键词	POSSIBLE PROBIOTIC TREATMENT ; SHRIMP PENAEUS-MONODON ; PSEUDOMONAS-FLUORESCENS ; AEROMONAS-SALMONICIDA ; VIBRIO-ANGUILLARUM ; FISH ; SPP. ; INHIBITION ; SURVIVAL ; GROWTH
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000301693600003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27559]
专题	自然资源部第一海洋研究所
通讯作者	Zheng, Fengrong
作者单位	1.First Inst Oceanog SOA, Ecol Lab, Qingdao 266061, Peoples R China 2.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fengrong,Liu, Hongzhan,Sun, Xiuqin,et al. Selection, identification and application of antagonistic bacteria associated with skin ulceration and peristome tumescence of cultured sea cucumber Apostichopus japonicus (Selenka)[J]. AQUACULTURE,2012,334:24-29.
APA	Zheng, Fengrong,Liu, Hongzhan,Sun, Xiuqin,Qu, Lingyun,Dong, Shuanglin,&Liu, Junling.(2012).Selection, identification and application of antagonistic bacteria associated with skin ulceration and peristome tumescence of cultured sea cucumber Apostichopus japonicus (Selenka).AQUACULTURE,334,24-29.
MLA	Zheng, Fengrong,et al."Selection, identification and application of antagonistic bacteria associated with skin ulceration and peristome tumescence of cultured sea cucumber Apostichopus japonicus (Selenka)".AQUACULTURE 334(2012):24-29.