HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root

doi:10.1016/j.jchromb.2007.11.041

	HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root
	Han, Chao 2; Shen, Yan 3; Chen, Junhui 1; Lee, Frank Sen-Chun 1; Wang, Xiaoru 1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2008-02
卷号	862 期号:1-2 页码:125-131
关键词	fingerprint chromatogram Pseudostellaria heterophylla (Miq.) pax HPLC-TOF-MS hierarchical clustering analysis similarity analysis cyclic peptides
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2007.11.041
英文摘要	High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (LC-TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC-TOF-MS analysis; one cyclic peptide was unequivocally identified and five cyclic peptides were tentatively assigned based on their MS data. These cyclic peptides served as the marker peaks in the HPLC fingerprints. The chromatographic fingerprints have been analyzed by similarity index calculations and hierarchical clustering analysis (HCA). The result showed that the HPLC fingerprints could be used to determine the optimal harvest time for R heterophylla (Miq.) Pax and to authenticate the species of the herb. (c) 2007 Elsevier B.V. All rights reserved.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; INHIBITORY CYCLIC-PEPTIDES ; FLIGHT MASS-SPECTROMETRY ; QUALITY-CONTROL ; L.
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000253354200014
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27535]
专题	自然资源部第一海洋研究所
通讯作者	Wang, Xiaoru
作者单位	1.First Inst Oceanog SOA, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China 2.Wenzhou Entry Exit Inspect & Quarantine Bur, Wenzhou 325027, Peoples R China 3.Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China
推荐引用方式 GB/T 7714	Han, Chao,Shen, Yan,Chen, Junhui,et al. HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,862(1-2):125-131.
APA	Han, Chao,Shen, Yan,Chen, Junhui,Lee, Frank Sen-Chun,&Wang, Xiaoru.(2008).HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,862(1-2),125-131.
MLA	Han, Chao,et al."HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 862.1-2(2008):125-131.