Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G | |
Che, Shuai1,2; Song, Weizhi1,2; Lin, Xuezheng1,2 | |
刊名 | CURRENT MICROBIOLOGY
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2013-11 | |
卷号 | 67期号:5页码:601-608 |
ISSN号 | 0343-8651 |
DOI | 10.1007/s00284-013-0409-3 |
英文摘要 | Temperature and salinity fluctuations are two of the most important factors affecting the growth of polar bacteria. In an attempt to better understand the function of heat-shock proteins (HSPs) in the adaptive mechanisms of the Antarctic psychrotrophic bacterium Psychrobacter sp. G to such conditions, genes Hsp845, Hsp2538, Hsp2666, and Hsp2667 were cloned on the basis of the draft genome. The expression characteristics of these HSP genes under different stress conditions were analyzed by the qRT-PCR method. Expression of Hsp845 and Hsp2667 was inhibited significantly by low temperature (0 and 10 A degrees C, respectively). There was no difference of expression when Hsp2538 and Hsp2666 were exposed to 0 A degrees C but the expression of Hsp2666 was inhibited when exposed to 10 A degrees C. Expression of Hsp2538 and Hsp2667 was not sensitive but expression of Hsp845 and Hsp2666 was increased at low salinity (0 and 15, respectively). Expression of the four HSP genes was enhanced at high salinity (90 and 120) and at high temperature independent of salinity. By contrast, low temperature had no significant effect independent of salinity. |
资助项目 | National Natural Science Foundation of China[41176174] |
WOS关键词 | ESCHERICHIA-COLI ; EXPRESSION ; GRPE ; DNAK ; EVOLUTIONARY ; INDUCTION ; CLONING ; GROEL ; PCR |
WOS研究方向 | Microbiology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000325225300014 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/27211] ![]() |
专题 | 自然资源部第一海洋研究所 |
通讯作者 | Che, Shuai |
作者单位 | 1.SOA, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.SOA, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Che, Shuai,Song, Weizhi,Lin, Xuezheng. Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G[J]. CURRENT MICROBIOLOGY,2013,67(5):601-608. |
APA | Che, Shuai,Song, Weizhi,&Lin, Xuezheng.(2013).Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G.CURRENT MICROBIOLOGY,67(5),601-608. |
MLA | Che, Shuai,et al."Response of Heat-Shock Protein (HSP) Genes to Temperature and Salinity Stress in the Antarctic Psychrotrophic Bacterium Psychrobacter sp G".CURRENT MICROBIOLOGY 67.5(2013):601-608. |
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