Discrimination of the common macroalgae (Ulva and Blidingia) in coastal waters of Yellow Sea, northern China, based on restriction fragment-length polymorphism (RFLP) analysis

doi:10.1016/j.hal.2013.05.003

	Discrimination of the common macroalgae (Ulva and Blidingia) in coastal waters of Yellow Sea, northern China, based on restriction fragment-length polymorphism (RFLP) analysis
	Xiao, Jie 1; Li, Yan 1; Song, Wei 1,2; Wang, Zongling 1; Fu, Mingzhu 1; Li, Ruixiang 1; Zhang, Xuelei 1; Zhu, Mingyuan 1
刊名	HARMFUL ALGAE
	2013-07
卷号	27 页码:130-137
关键词	Green tides Ulva prolifera Genetic identification RFLP Porphyra aquaculture Yellow Sea
ISSN号	1568-9883
DOI	10.1016/j.hal.2013.05.003
英文摘要	Since 2007, reoccurring large-scale green algae blooms have caused deleterious effects to the estuarine ecosystem of Yellow Sea, northern China and subsequent economical losses. Previous surveys indicated the green tides were initiated in the coastal water of southern Jiangsu province where Porphyra farming was intensively conducted; however, the main 'seed source' of floating green algae is still under debate. Ulva prolifera was confirmed to be the major causative species of green tides. The multiple sympatric ulvoid species in the natural environment has complicated species identification in both field surveys and laboratory studies due to their morphological plasticity. Thus, we developed a genetic identification key based on restriction fragment length polymorphism (RFLP) analysis of the ITS nuclear marker to discriminate the common Ulva and Blidingia species in the Yellow Sea. Ten genetic lineages (1 in Blidingia, 9 in Ulva) were detected along the coast of China through phylogenetic analysis of ITS sequences. They can be separated by virtual restriction digestion using the four selected restriction enzymes (BspT107 I, Eco0109 I, Hin1 I and VpaK11B I). With additional PCR amplification of the 5S spacer region, we were able to discriminate U. prolifera from Ulva linza. Using this genetic key, we screened macroalgal samples collected from the coast of the Yellow Sea, and the results indicated 6 common lineages (U. prolifera, U. linza, Ulva compressa, Ulva pertusa, Clade 6 and Blidingia sp.) in this region, which could be explicitly distinguished by a single enzyme (BspT107 I) coupled with 5S spacer polymorphism. U. prolifera was confirmed to be present on the Porphyra aquaculture rafts with seasonal variation in the species composition. This genetic key will facilitate our long-term field surveys to investigate the origin of the floating U. prolifera and furthermore to explore its bloom dynamics along the coast of the Yellow Sea. It also provided a framework for the future inclusion of more Ulva species, which will expand the usage of this key. (C) 2013 Elsevier B.V. All rights reserved.
资助项目	Qingdao Municipal Science and Technology plan project[11-3-1-7-hy]
WOS关键词	SCALE GREEN TIDE ; SEAWEED AQUACULTURE ; PORPHYRA-YEZOENSIS ; MOLECULAR ANALYSIS ; FRESH-WATER ; ENTEROMORPHA ; CHLOROPHYTA ; ULVOPHYCEAE ; BLOOM ; MORPHOLOGY
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000322298400012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27172]
专题	自然资源部第一海洋研究所
通讯作者	Li, Yan
作者单位	1.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Jie,Li, Yan,Song, Wei,et al. Discrimination of the common macroalgae (Ulva and Blidingia) in coastal waters of Yellow Sea, northern China, based on restriction fragment-length polymorphism (RFLP) analysis[J]. HARMFUL ALGAE,2013,27:130-137.
APA	Xiao, Jie.,Li, Yan.,Song, Wei.,Wang, Zongling.,Fu, Mingzhu.,...&Zhu, Mingyuan.(2013).Discrimination of the common macroalgae (Ulva and Blidingia) in coastal waters of Yellow Sea, northern China, based on restriction fragment-length polymorphism (RFLP) analysis.HARMFUL ALGAE,27,130-137.
MLA	Xiao, Jie,et al."Discrimination of the common macroalgae (Ulva and Blidingia) in coastal waters of Yellow Sea, northern China, based on restriction fragment-length polymorphism (RFLP) analysis".HARMFUL ALGAE 27(2013):130-137.