Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination

doi:10.1111/j.1365-2109.2009.02435.x

	Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination
	Zheng, Feng Rong 1; Sun, Xiu Qin 1; Xing, Ming Qing 1; Liu, Hongzhan 2
刊名	AQUACULTURE RESEARCH
	2010-09-16
卷号	41 期号:10 页码:1444-1451
关键词	Japanese flounder lymphocystis disease virus DNA vaccine major histocompatibility class TCR immune-related gene
ISSN号	1355-557X
DOI	10.1111/j.1365-2109.2009.02435.x
英文摘要	In this study, we found that an intramuscular injection of Japanese flounder (Paralichthys olivaceus, 60-80 g in weight and 15-20 mL in length) with 5 mu g of a DNA vaccine (pEGFP-N2-LCDV-cn-MCP 0.6 kb, containing lymphocystis disease virus major capsid protein gene) induced a strong immune response. Subsequent real-time polymerase chain reaction showed that the expression of immune-related genes [e.g., major histocompatibility complex (MHC) class I alpha, MHC II alpha, T-cell receptor (TCR), tumour necrosis factor (TNF), tumour necrosis factor receptor (TNFR), Mx, interleukin (IL)-1 beta, CXC and IL-8R] was significantly changed after DNA vaccination. The most remarkable alternation was the expression of MHC I alpha and MHC II alpha genes: MHC II alpha reached the maximum on day 8 in different tissues, and MHC I alpha on day 2 in the intestine and gills. The expression of TCR increased and reached a plateau in 2 days in the spleen, gills, kidney and liver after vaccination and then decreased after day 8. In contrast, the expression of TCR in the intestine increased and reached a plateau in 8 days. The expression of IL-8R reached the maximum on day 2 in different tissues and then decreased on day 8. Mx increased in the gills, kidney, spleen and liver on days 2, 8, 2 and 2, but decreased in the intestine, gills, spleen and liver on days 2, 8, 8 and 8 respectively. The TNFR expression increased in the spleen, kidney and gills on days 2, 8 and 8, but decreased in intestine, liver and gills on days 2, 8 and 8 respectively. The expression of TNF, CXC and IL-1 beta increased 2 and 8 days after the injection of DNA vaccine. However, the expression of TNF, CXC and IL-1 beta altered on days 2 and 8 with different patterns in different tissues respectively. The fish responded to the DNA vaccine by yielding a specific immunoglobulin against lymphocystis disease virus (LCDV) as observed with indirect ELISA. The DNA vaccine induced a unique humoral response, suggesting that the DNA vaccine activated both cellular and humoral defences of the specific immune system of Japanese flounder.
资助项目	National High-tech Research and Development (863) Program of China[2003AA622030] ; National High-tech Research and Development (863) Program of China[2006AA100309]
WOS关键词	TROUT ONCORHYNCHUS-MYKISS ; HEMATOPOIETIC NECROSIS VIRUS ; HEMORRHAGIC SEPTICEMIA VIRUS ; FLOUNDER PARALICHTHYS-OLIVACEUS ; INFECTIOUS PANCREATIC NECROSIS ; SALMON SALMO-SALAR ; MHC CLASS-I ; RAINBOW-TROUT ; PROTECTIVE IMMUNITY ; ATLANTIC SALMON
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000282178000044
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27160]
专题	自然资源部第一海洋研究所
通讯作者	Sun, Xiu Qin
作者单位	1.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.Shandong Univ, Marine Coll, Weihai, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Feng Rong,Sun, Xiu Qin,Xing, Ming Qing,et al. Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination[J]. AQUACULTURE RESEARCH,2010,41(10):1444-1451.
APA	Zheng, Feng Rong,Sun, Xiu Qin,Xing, Ming Qing,&Liu, Hongzhan.(2010).Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination.AQUACULTURE RESEARCH,41(10),1444-1451.
MLA	Zheng, Feng Rong,et al."Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination".AQUACULTURE RESEARCH 41.10(2010):1444-1451.