Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea

doi:10.1093/femsle/fnw086

	Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea
	He, Peiqing 1; Li, Li 2; Liu, Jihua 2; Bai, Yazhi 2; Fang, Xisheng 2
刊名	FEMS MICROBIOLOGY LETTERS
	2016-05
卷号	363 期号:10
关键词	Catechol 2, 3-dioxygenase diversity aromatic hydrocarbons the Bohai Sea
ISSN号	0378-1097
DOI	10.1093/femsle/fnw086
英文摘要	Catechol 2, 3-dioxygenase (C23O) is the key enzyme for aerobic aromatic degradation. Based on clone libraries and quantitative real-time polymerase chain reaction, we characterized diversity and distribution patterns of C23O genes in surface sediments of the Bohai Sea. The results showed that sediments of the Bohai Sea were dominated by genes related to C23O subfamily I. 2. A. The samples from wastewater discharge area (DG) and aquaculture farm (KL) showed distinct composition of C23O genes when compared to the samples from Bohai Bay (BH), and total organic carbon was a crucial determinant accounted for the composition variation. C6BH12-38 and C2BH2-35 displayed the highest gene copies and highest ratios to the 16S rRNA genes in KL, and they might prefer biologically labile aromatic hydrocarbons via aquaculture inputs. Meanwhile, C7BH3-48 showed the highest gene copies and highest ratios to the 16S rRNA genes in DG, and this could be selective effect of organic loadings from wastewater discharge. An evident increase in C6BH12-38 and C7BH3-48 gene copies and reduction in diversity of C23O genes in DG and KL indicated composition perturbations of C23O genes and potential loss in functional redundancy. We suggest that ecological habitat and trophic specificity could shape the distribution of C23O genes in the Bohai Sea sediments.
资助项目	Public science and technology research funds projects of ocean[201105003]
WOS关键词	POLYCYCLIC AROMATIC-HYDROCARBONS ; ORGANIC-MATTER SOURCE ; COMMUNITY COMPOSITION ; CATABOLIC GENES ; BACTERIA ; LAKE ; BACTERIOPLANKTON ; DEGRADATION ; CHINA ; SOIL
WOS研究方向	Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000377970800004
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27089]
专题	自然资源部第一海洋研究所
通讯作者	Liu, Jihua
作者单位	1.Marine Ecol Res Ctr, State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab State Ocean Adm Marine Sedimentol & Envir, 6 Xianxialing Rd, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	He, Peiqing,Li, Li,Liu, Jihua,et al. Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea[J]. FEMS MICROBIOLOGY LETTERS,2016,363(10).
APA	He, Peiqing,Li, Li,Liu, Jihua,Bai, Yazhi,&Fang, Xisheng.(2016).Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea.FEMS MICROBIOLOGY LETTERS,363(10).
MLA	He, Peiqing,et al."Diversity and distribution of catechol 2, 3-dioxygenase genes in surface sediments of the Bohai Sea".FEMS MICROBIOLOGY LETTERS 363.10(2016).