A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp ICE-L

doi:10.1007/s10126-010-9309-8

	A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp ICE-L
	Zhang, Pengying 2,3; Liu, Shenghao 1; Cong, Bailin 1; Wu, Guangting 1; Liu, Chenlin 1; Lin, Xuezheng 1; Shen, Jihong 1; Huang, Xiaohang 1
刊名	MARINE BIOTECHNOLOGY
	2011-06
卷号	13 期号:3 页码:393-401
关键词	Antarctic ice algae Chlamydomonas sp ICE-L Omega-3 fatty acid desaturase Psychrophile Stress
ISSN号	1436-2228
DOI	10.1007/s10126-010-9309-8
英文摘要	The ability of Antarctic ice algae, Chlamydomonas sp. ICE-L, to survive and proliferate at low temperature and high salinity implies that they have overcome key barriers inherent in Antarctic environments. A full-length complementary DNA (cDNA) sequence of omega-3 fatty acid desaturase, designated CiFAD3, was isolated via reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The full-length of CiFAD3 cDNA contained an open reading frame of 1,302 bp with 5'-terminal untranslated region (UTR) of 36 bp and 3'-terminal UTR of 507 bp encoding a fatty acid desaturase protein of 434 amino acids. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known chloroplastic omega-3 fatty acid desaturase. Meanwhile, CiFAD3 sequence showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane spanning regions that were universally present among plant desaturases. Under different stress conditions, messenger RNA (mRNA) expression levels of CiFAD3 were measured by quantitative RT-PCR. The results showed that both temperature and salinity could motivate the upregulation of CiFAD3 expression. The mRNA accumulation of CiFAD3 increased 2.6-fold at 0A degrees C and 1.8-fold at 12A degrees C compared to the algae at 6A degrees C. Similarly, mRNA expression levels of CiFAD3 increased 3.8-fold after 62aEuro degrees NaCl treatment for 2 h. However, CiFAD3 mRNA expression levels were partially decreased after UV radiation. These data suggest that CiFAD3 is the enzyme responsible for the omega-3 fatty acid desaturation involved in ice algae Chlamydomonas sp. ICE-L acclimatizing to cold temperature and high salinity in Antarctic environment.
资助项目	China 863 High-Technology Key Projects[2007AA091905]
WOS关键词	MEMBRANE-FLUIDITY ; LIPID-COMPOSITION ; GENE-EXPRESSION ; ACID ; MICROBIOLOGY ; CLONING ; STRESS ; FUNGI
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000291168500005
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26601]
专题	自然资源部第一海洋研究所
通讯作者	Huang, Xiaohang
作者单位	1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Shandong, Peoples R China 2.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 3.Shandong Univ, Coll Life Sci, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Pengying,Liu, Shenghao,Cong, Bailin,et al. A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp ICE-L[J]. MARINE BIOTECHNOLOGY,2011,13(3):393-401.
APA	Zhang, Pengying.,Liu, Shenghao.,Cong, Bailin.,Wu, Guangting.,Liu, Chenlin.,...&Huang, Xiaohang.(2011).A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp ICE-L.MARINE BIOTECHNOLOGY,13(3),393-401.
MLA	Zhang, Pengying,et al."A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp ICE-L".MARINE BIOTECHNOLOGY 13.3(2011):393-401.