Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil

doi:10.1007/s10126-018-9850-4

	Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil
	Chen, Ze 1; Zheng, Zhou 2,3; Wang, Feng-Lian 1; Niu, Yuan-Pu 1; Miao, Jin-Lai 2,3; Li, Hao 1
刊名	MARINE BIOTECHNOLOGY
	2018-12
卷号	20 期号:6 页码:803-812
关键词	Diesel oil biodegradation Rhodococcus sp LH Molecular mechanisms Metabolomics
ISSN号	1436-2228
DOI	10.1007/s10126-018-9850-4
英文摘要	In recent years, some marine microbes have been used to degrade diesel oil. However, the exact mechanisms underlying the biodegradation are still poorly understood. In this study, a hypothermophilous marine strain, which can degrade diesel oil in cold seawater was isolated from Antarctic floe-ice and identified and named as Rhodococcus sp. LH. To clarify the biodegradation mechanisms, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics strategy was performed to determine the diesel biodegradation process-associated intracellular biochemical changes in Rhodococcus sp. LH cells. With the aid of partial least squares-discriminant analysis (PLS-DA), 17 differential metabolites with variable importance in the projection (VIP) value greater than 1 were identified. Results indicated that the biodegradation of diesel oil by Rhodococcus sp. LH was affected by many different factors. Rhodococcus sp. LH could degrade diesel oil through terminal or sub-terminal oxidation reactions, and might also possess the ability to degrade aromatic hydrocarbons. In addition, some surfactants, especially fatty acids, which were secreted by Rhodococcus into medium could also assist the strain in dispersing and absorbing diesel oil. Lack of nitrogen in the seawater would lead to nitrogen starvation, thereby restraining the amino acid circulation in Rhodococcus sp. LH. Moreover, nitrogen starvation could also promote the conversation of relative excess carbon source to storage materials, such as 1-monolinoleoylglycerol. These results would provide a comprehensive understanding about the complex mechanisms of diesel oil biodegradation by Rhodococcus sp. LH at the systematic level.
资助项目	National Natural Science Foundation of China[41776203]
WOS关键词	NAPHTHALENE DIOXYGENASE ; N-ALKANES ; HYDROCARBONS ; DEGRADATION ; OXIDATION ; RESPONSES ; PATHWAY ; CARBON ; ACIDS ; RHA1
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000451022400010
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26535]
专题	自然资源部第一海洋研究所
通讯作者	Miao, Jin-Lai; Li, Hao
作者单位	1.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266235, Peoples R China
推荐引用方式 GB/T 7714	Chen, Ze,Zheng, Zhou,Wang, Feng-Lian,et al. Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil[J]. MARINE BIOTECHNOLOGY,2018,20(6):803-812.
APA	Chen, Ze,Zheng, Zhou,Wang, Feng-Lian,Niu, Yuan-Pu,Miao, Jin-Lai,&Li, Hao.(2018).Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil.MARINE BIOTECHNOLOGY,20(6),803-812.
MLA	Chen, Ze,et al."Intracellular Metabolic Changes of Rhodococcus sp LH During the Biodegradation of Diesel Oil".MARINE BIOTECHNOLOGY 20.6(2018):803-812.