The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China

doi:10.1016/j.bjm.2016.09.007

	The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
	Yao, Xie-Feng 1; Zhang, Jiu-ming 3,4; Tian, Li 3,4; Guo, Jian-hua 2
刊名	BRAZILIAN JOURNAL OF MICROBIOLOGY
	2017
卷号	48 期号:1 页码:71-78
关键词	Heavy metal contamination PCR-DGGE fingerprinting Multivariate analysis Bacterial community structure
ISSN号	1517-8382
DOI	10.1016/j.bjm.2016.09.007
英文摘要	In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
资助项目	Fundamental Research Funds forhangsu Academy of Agricultural Sciences[ZX(15)4007]
WOS关键词	GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; MICROBIAL COMMUNITY ; ENVIRONMENTAL GRADIENTS ; EUKARYOTIC COMMUNITIES ; NORTH CHINA ; LAKE DONGHU ; DIVERSITY ; GENES ; SEDIMENTS
WOS研究方向	Microbiology
语种	英语
出版者	SOC BRASILEIRA MICROBIOLOGIA
WOS记录号	WOS:000394144100014
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26430]
专题	自然资源部第一海洋研究所
通讯作者	Tian, Li; Guo, Jian-hua
作者单位	1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Inst Vegetable Crops, Nanjing, Jiangsu, Peoples R China 2.Nanjing Agr Univ, Dept Plant Pathol, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Qingdao, Peoples R China 4.Qingdao Univ Sci & Technol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yao, Xie-Feng,Zhang, Jiu-ming,Tian, Li,et al. The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China[J]. BRAZILIAN JOURNAL OF MICROBIOLOGY,2017,48(1):71-78.
APA	Yao, Xie-Feng,Zhang, Jiu-ming,Tian, Li,&Guo, Jian-hua.(2017).The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China.BRAZILIAN JOURNAL OF MICROBIOLOGY,48(1),71-78.
MLA	Yao, Xie-Feng,et al."The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China".BRAZILIAN JOURNAL OF MICROBIOLOGY 48.1(2017):71-78.