Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches

doi:10.1016/j.compbiolchem.2007.08-002

	Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches
	Chen, Kui ; Huang, Xiaohang
刊名	COMPUTATIONAL BIOLOGY AND CHEMISTRY
	2007-10-01
卷号	31 期号:5-6 页码:378-383
关键词	sea perch SNARE proteins motif 7S core complex protein structure analysis
ISSN号	1476-9271
DOI	10.1016/j.compbiolchem.2007.08-002
英文摘要	Three cDNA sequences encoding four SNARE (N-ethylmaleimide-sensitive fusion protein attachment protein receptors) motifs were cloned from sea perch, and the deduced peptide sequences were analyzed for structural prediction by using 14 different web servers and softwares. The "ionic layer" structure, the three dimensional extension and conformational characters of the SNARE 7S core complex by using bioinformatics approaches were compared respectively with those from mammalian X-ray crystallographic investigations. The result suggested that the formation and stabilization of fish SNARE core complex might be driven by hydrophobic association, hydrogen bond among R group of core amino acids and electrostatic attraction at molecular level. This revealed that the SNARE proteins interaction of the fish may share the same molecular mechanism with that of mammal, indicating the universality and solidity of SNARE core complex theory. This work is also an attempt to get the protein 3D structural information which appears to be similar to that obtained through X-ray crystallography, only by using computerized approaches. (C) 2007 Elsevier Ltd. All rights reserved.
WOS关键词	SECONDARY STRUCTURE PREDICTION ; MEMBRANE-FUSION ; SEQUENCE-ANALYSIS ; CRYSTAL-STRUCTURE ; PROTEIN-SEQUENCE ; BLOCKS DATABASE ; EXOCYTOSIS ; COMPLEX ; INSIGHTS ; SERVER
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Computer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000250912400009
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26320]
专题	自然资源部第一海洋研究所
通讯作者	Huang, Xiaohang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen, Kui,Huang, Xiaohang. Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches[J]. COMPUTATIONAL BIOLOGY AND CHEMISTRY,2007,31(5-6):378-383.
APA	Chen, Kui,&Huang, Xiaohang.(2007).Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches.COMPUTATIONAL BIOLOGY AND CHEMISTRY,31(5-6),378-383.
MLA	Chen, Kui,et al."Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches".COMPUTATIONAL BIOLOGY AND CHEMISTRY 31.5-6(2007):378-383.