Starvation effects on pathogenic Vibrio alginolyticus in natural seawater

	Starvation effects on pathogenic Vibrio alginolyticus in natural seawater
	Yi Jiabo 1; Chen Qiang 1; Zou Wenzheng 1; Yan Qingpi 1; Zhuang Zhixia 2; Wang Xiaoru 2
刊名	ACTA OCEANOLOGICA SINICA
	2008
卷号	27 期号:4 页码:120-129
关键词	Vibrio alginolyticus starvation survival adhesion ELISA resistance
ISSN号	0253-505X
英文摘要	To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus, log-phase cells were inoculated into sterile natural seawater for starvation studies. The results showed that all of total bacteria number, viable bacteria number and CFU number of V. alginolyticus increased remarkably at the initial starvation stage; after reaching their peaks at 5 d, both total bacteria number and viable bacteria number of V. alginolyticus fell slowly, while the CFU Muni er fell more quickly after reaching its peak at 10 d; V. alginolyticus elongated their cells at the prophase of starvation, and their shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation; starved cells showed more sensitivity to heating and UV; starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay (ELISA); starved cells' ability to adhere to the skin mucus of large yellow croaker (Pseudosciaena crocea) showed a sharp decline as tire starvation time increases; the cellular protein of V. alginolyticus increased remarkably at the anaphase of starvation. The results indicated that pathogenic 1 alginolyticus could survive in starvation for relatively long periods of time (>= 2 months) in 28 degrees C natural seawater due to the morphological and physiological changes; however, starved 1 alginolyticus cells showed less virulence and higher sensitivity under environmental stresses.
资助项目	Natural Science Foundation of Fujian Province of China[B0410022]
WOS关键词	NONCULTURABLE STATE ; LOW-TEMPERATURE ; CELLS ; SEA ; MICROORGANISMS ; VULNIFICUS ; SURVIVAL ; BACTERIA ; PARAHAEMOLYTICUS ; RESUSCITATION
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000260878500011
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26294]
专题	自然资源部第一海洋研究所
通讯作者	Yan Qingpi
作者单位	1.Jimei Univ, Coll Fisheries, Key Lab Sci & Technol Aquaculture & Food Safety, Xiamen 361021, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Yi Jiabo,Chen Qiang,Zou Wenzheng,et al. Starvation effects on pathogenic Vibrio alginolyticus in natural seawater[J]. ACTA OCEANOLOGICA SINICA,2008,27(4):120-129.
APA	Yi Jiabo,Chen Qiang,Zou Wenzheng,Yan Qingpi,Zhuang Zhixia,&Wang Xiaoru.(2008).Starvation effects on pathogenic Vibrio alginolyticus in natural seawater.ACTA OCEANOLOGICA SINICA,27(4),120-129.
MLA	Yi Jiabo,et al."Starvation effects on pathogenic Vibrio alginolyticus in natural seawater".ACTA OCEANOLOGICA SINICA 27.4(2008):120-129.