Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process

doi:10.1039/c6ra19254h

	Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process
	Chen, Ze 1; Zheng, Zhou 2; Yi, Chenfeng 1; Wang, Fenglian 1; Niu, Yuanpu 1; Li, Hao 1
刊名	RSC ADVANCES
	2016
卷号	6 期号:107 页码:105046-105055
ISSN号	2046-2069
DOI	10.1039/c6ra19254h
英文摘要	During the batch bioethanol fermentation process, although Saccharomyces cerevisiae cells are challenged by accumulated ethanol, our previous work showed that the ethanol tolerance of S. cerevisiae increased as fermentation time increased. However, the exact molecular mechanisms underlying the increased ethanol tolerance of S. cerevisiae are still poorly understood. In this study, a gas chromatography-mass spectrometry-based metabolomics strategy was used to determine the fermentation process-associated intracellular metabolic changes in S. cerevisiae cells. With the aid of partial least squares-discriminant analysis between two of the three fermentation stages (i.e., the lag, exponential, and stationary phases), 40 differential metabolites with variable importance and a projection value greater than 1 were identified. During the bioethanol fermentation process, S. cerevisiae cells could continuously remodel their membrane composition and structure to obtain higher ethanol tolerance. During the lag-exponential phase transition, in spite of a down-regulated TCA cycle, the increased ergosterol content combined with decreased saturated fatty acid content might be the most significant factor in making yeast cells more robust and ethanol-tolerant. During the exponential-stationary phase transition, a re-activated TCA cycle could provide plenty of energy, and the increased energy production together with the increased energy requirements might be partly responsible for the increased ethanol tolerance in the stationary phase. Moreover, the increased content of glycerol, trehalose, ergosterol and some amino acids also might jointly confer the yeast cells with higher ethanol tolerance. These results highlighted our knowledge about the relationship between the bioethanol fermentation process and ethanol tolerance, and could contribute to the construction of feasible ethanologenic strains with higher ethanol tolerance.
资助项目	Fundamental Research Funds of the First Institute of Oceanography, SOA[2013G32]
WOS关键词	TREHALOSE ACCUMULATION ; SYNTHASE TPS1 ; YEAST STRAINS ; BUDDING YEAST ; STRESS ; DEHYDROGENASE ; MEMBRANE ; GENES ; OSMOADAPTATION ; RESPONSES
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000388116500013
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25909]
专题	自然资源部第一海洋研究所
通讯作者	Li, Hao
作者单位	1.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China 2.SOA, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Chen, Ze,Zheng, Zhou,Yi, Chenfeng,et al. Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process[J]. RSC ADVANCES,2016,6(107):105046-105055.
APA	Chen, Ze,Zheng, Zhou,Yi, Chenfeng,Wang, Fenglian,Niu, Yuanpu,&Li, Hao.(2016).Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process.RSC ADVANCES,6(107),105046-105055.
MLA	Chen, Ze,et al."Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process".RSC ADVANCES 6.107(2016):105046-105055.