Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus

doi:10.1016/j.cbd.2017.02.003

	Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus
	Sun, Lina 1; Sun, Jingchun 1; Xu, Qinzeng 3; Li, Xiaoni 1,2; Zhang, Libin 1; Yang, Hongsheng 1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2017-06
卷号	22 页码:32-38
关键词	Sea cucumber Metabolic responses Gene expression Intestine regeneration
ISSN号	1744-117X
DOI	10.1016/j.cbd.2017.02.003
英文摘要	Sea cucumbers are excellent models for studying organ regeneration due to their striking capacity to regenerate most of their viscera after evisceration. In this study, we applied NMR-based metabolomics to determine the metabolite changes that occur during the process of intestine regeneration in sea cucumbers. Partial least-squares discriminant analysis showed that there was significant differences in metabolism between regenerative intestines at 3, 7, and 14 days post evisceration (dpe) and normal intestines. Changes in the concentration of 13 metabolites related to regeneration were observed and analyzed. These metabolites included leucine, isoleucine, valine, arginine, glutamate, hypotaurine, dimethylamine, N,N-dimethylglycine, betaine, taurine, inosine, homarine, and histidine. Three important genes (betaine-aldehyde dehydrogenase, betaine-homocysteine S-methyltransferase 1, and dimethylglycine dehydrogenase) were differentially expressed to regulate the levels of betaine and N,N-dimethylglycine during intestine regeneration. These results provide an important basis for studying regenerative mechanisms and developing regenerative matrixes. (C) 2017 Elsevier Inc. All rights reserved.
资助项目	Chinese Academy of Sciences[XDA11020703]
WOS关键词	ECHINODERM HOLOTHURIA-GLABERRIMA ; CENTRAL-NERVOUS-SYSTEM ; VISCERAL REGENERATION ; DEHYDROGENASE GENE ; EXPRESSION ; TAURINE ; DEDIFFERENTIATION ; PROLIFERATION ; INVOLVEMENT ; NUTRITION
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000402220500004
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25838]
专题	自然资源部第一海洋研究所
通讯作者	Zhang, Libin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Ecol & Environm Sci & Engn, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lina,Sun, Jingchun,Xu, Qinzeng,et al. Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:32-38.
APA	Sun, Lina,Sun, Jingchun,Xu, Qinzeng,Li, Xiaoni,Zhang, Libin,&Yang, Hongsheng.(2017).Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,32-38.
MLA	Sun, Lina,et al."Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):32-38.