Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS

doi:10.1365/s10337-007-0333-4

	Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS
	Shen, Yan ; Han, Chao ; Chen, Junhui ; Wan, Xiaoru
刊名	CHROMATOGRAPHIA
	2007-09
卷号	66 期号:5-6 页码:319-323
关键词	column liquid chromatography HPLC-APCI-MS Traditional Chinese Medicine cyclic peptides Pseudostellaria heterophylla (Miq.) Pax
ISSN号	0009-5893
DOI	10.1365/s10337-007-0333-4
英文摘要	A new method for the analysis of cyclic peptides (Pseudostellarins) in traditional Chinese medicine, Pseudostellaria heterophylla (Miq.) Pax, was established by high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). The real samples were separated by a reversed-phase C-18 column using a binary eluent under gradient conditions. Six cyclic peptides were isolated and detected with a DAD detector. The Pseudostellarins components were identified by APCI-MS in a negative mode. in the negative ion APCI mode, the best sensitivity and the lowest detection limit for Pseudostellarins were obtained by using a mobile phase consisting of acetonitrile and pure water. The APCI spectra were characterized by [M-H](-) peak. The parent ions [M-H](-) with highest intensity was used for quantification of one cyclic peptide-Pseudostellarin B.
WOS关键词	CHROMATOGRAPHY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000249712300003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25828]
专题	自然资源部第一海洋研究所
通讯作者	Shen, Yan
作者单位	1.Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China 2.Wenzhou Entry Exit Inspect & Quarantine Bur, Wenzhou 325027, Peoples R China 3.Inst Oceanog 1, Qingdao Key Lab Analyt Tech Dev & Standardizat Ch, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Shen, Yan,Han, Chao,Chen, Junhui,et al. Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS[J]. CHROMATOGRAPHIA,2007,66(5-6):319-323.
APA	Shen, Yan,Han, Chao,Chen, Junhui,&Wan, Xiaoru.(2007).Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS.CHROMATOGRAPHIA,66(5-6),319-323.
MLA	Shen, Yan,et al."Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS".CHROMATOGRAPHIA 66.5-6(2007):319-323.