The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza | |
Dong, Meitao2; Zhang, Xiaowen1; Chi, Xiaoyuan3; Mou, Shanli4; Xu, Jianfang4; Xu, Dong1; Wang, Wenqi2; Ye, Naihao1 | |
刊名 | CURRENT GENETICS
![]() |
2012-02 | |
卷号 | 58期号:1页码:13-20 |
关键词 | Ulva linza RT-qPCR Validation Reference genes |
ISSN号 | 0172-8083 |
DOI | 10.1007/s00294-011-0361-3 |
英文摘要 | Normalization based on inappropriate reference gene may lead to the reduction of the accuracy of RT-qPCR. Although determination of suitable reference genes is essential to RT-qPCR studies, reports on the evaluation of reference genes in Ulva linza, a ubiquitous green-tide forming alga, are lacking. The expression levels of ten candidate reference genes were analyzed in U. linza across different experimental treatments, and the best-ranked reference genes differed across the treatments. The most suitable reference genes were tubulin2 (TUB2) among different salinity and UV treatments. Histone 2 (H2) was stably expressed in different temperature and desiccation stress treatments. 18S rRNA exhibited better expression stability in different light intensity treatments. While all tested samples were considered, none of single gene was widely applicable as a reference gene. Moreover, using a combination of two genes as reference genes might improve the reliability of gene expression by RT-qPCR, and the combination of TUB1 and TUB2 was selected as ideal for all tested samples. The results suggest that assessing the stability of reference gene expression patterns, determining candidates, and testing their suitability are required for each experimental investigation. The results will guide the selection of reference genes for gene expression studies in U. linza. |
资助项目 | National Science & Technology Pillar Program[2008BAD95B11] |
WOS关键词 | TIME RT-PCR ; POLYMERASE-CHAIN-REACTION ; SYSTEMATIC VALIDATION ; HOUSEKEEPING GENES ; ABIOTIC STRESS ; NORMALIZATION ; EXPRESSION ; SELECTION ; RNA ; QUANTIFICATION |
WOS研究方向 | Genetics & Heredity |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000302064600002 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/25820] ![]() |
专题 | 自然资源部第一海洋研究所 |
通讯作者 | Ye, Naihao |
作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China 3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China 4.State Ocean Adm SOA, Inst Oceanog 1, Key Lab Marine Bioact Substance, Qingdao 266061, Peoples R China |
推荐引用方式 GB/T 7714 | Dong, Meitao,Zhang, Xiaowen,Chi, Xiaoyuan,et al. The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza[J]. CURRENT GENETICS,2012,58(1):13-20. |
APA | Dong, Meitao.,Zhang, Xiaowen.,Chi, Xiaoyuan.,Mou, Shanli.,Xu, Jianfang.,...&Ye, Naihao.(2012).The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza.CURRENT GENETICS,58(1),13-20. |
MLA | Dong, Meitao,et al."The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza".CURRENT GENETICS 58.1(2012):13-20. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论