The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza

doi:10.1007/s00294-011-0361-3

	The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza
	Dong, Meitao 2; Zhang, Xiaowen 1; Chi, Xiaoyuan 3; Mou, Shanli 4; Xu, Jianfang 4; Xu, Dong 1; Wang, Wenqi 2; Ye, Naihao 1
刊名	CURRENT GENETICS
	2012-02
卷号	58 期号:1 页码:13-20
关键词	Ulva linza RT-qPCR Validation Reference genes
ISSN号	0172-8083
DOI	10.1007/s00294-011-0361-3
英文摘要	Normalization based on inappropriate reference gene may lead to the reduction of the accuracy of RT-qPCR. Although determination of suitable reference genes is essential to RT-qPCR studies, reports on the evaluation of reference genes in Ulva linza, a ubiquitous green-tide forming alga, are lacking. The expression levels of ten candidate reference genes were analyzed in U. linza across different experimental treatments, and the best-ranked reference genes differed across the treatments. The most suitable reference genes were tubulin2 (TUB2) among different salinity and UV treatments. Histone 2 (H2) was stably expressed in different temperature and desiccation stress treatments. 18S rRNA exhibited better expression stability in different light intensity treatments. While all tested samples were considered, none of single gene was widely applicable as a reference gene. Moreover, using a combination of two genes as reference genes might improve the reliability of gene expression by RT-qPCR, and the combination of TUB1 and TUB2 was selected as ideal for all tested samples. The results suggest that assessing the stability of reference gene expression patterns, determining candidates, and testing their suitability are required for each experimental investigation. The results will guide the selection of reference genes for gene expression studies in U. linza.
资助项目	National Science & Technology Pillar Program[2008BAD95B11]
WOS关键词	TIME RT-PCR ; POLYMERASE-CHAIN-REACTION ; SYSTEMATIC VALIDATION ; HOUSEKEEPING GENES ; ABIOTIC STRESS ; NORMALIZATION ; EXPRESSION ; SELECTION ; RNA ; QUANTIFICATION
WOS研究方向	Genetics & Heredity
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000302064600002
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25820]
专题	自然资源部第一海洋研究所
通讯作者	Ye, Naihao
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China 3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China 4.State Ocean Adm SOA, Inst Oceanog 1, Key Lab Marine Bioact Substance, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Dong, Meitao,Zhang, Xiaowen,Chi, Xiaoyuan,et al. The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza[J]. CURRENT GENETICS,2012,58(1):13-20.
APA	Dong, Meitao.,Zhang, Xiaowen.,Chi, Xiaoyuan.,Mou, Shanli.,Xu, Jianfang.,...&Ye, Naihao.(2012).The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza.CURRENT GENETICS,58(1),13-20.
MLA	Dong, Meitao,et al."The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza".CURRENT GENETICS 58.1(2012):13-20.