Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS

doi:10.1365/s10337-009-1152-6

	Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS
	Shen, Yan 2; Liu, Aili 2; Ye, Mingde 2; Wang, Li 3; Chen, Junhui 4; Wang, Xiao-ru 4; Han, Chao 1
刊名	CHROMATOGRAPHIA
	2009-08
卷号	70 期号:3-4 页码:431-438
关键词	Column liquid chromatography-mass spectrometry Microwave assisted extraction LC-DAD-ESI-MSn LC-ESI-TOF-MS Triterpene saponins Centella asiatica
ISSN号	0009-5893
DOI	10.1365/s10337-009-1152-6
英文摘要	Centella asiatica (L.) Urban is a traditional herbal medicine used in Asiatic countries, and is commonly used to treat various wounds, leprosy, tuberculosis and lupus diseases. In this work, a new method based on microwave assisted extraction followed by liquid chromatography-diode array detection-electrospray ionization multistage tandem mass spectrometry analysis has been developed for the identification and quantification of biologically active constituents in C. asiatica, including asiatic acid, asiaticoside and madecassoside. The separation was performed on an Agilent Eclipse C-18 column (150 mm x 4.6 mm i.d., 5 mu m) with gradient elution of acetonitrile and 0.1% aqueous acetic acid within 50 min. Detection was performed at 205 nm. The calibration curves showed good linearity (r (2) > 0.9992). The limits of detection ranged from 1.2 to 1.6 mu g mL(-1). The intra- and inter-day precision was less than 3% and the recovery of the assay was in the range of 95.4-106.8%. The method was successfully applied to the quantification of the three constituents in different samples of C. asiatica. The results indicated that the developed method could be considered to be a simple, rapid and reliable method for the quality evaluation of C. asiatica. The samples were also analyzed on a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry system to confirm the identification results.
资助项目	Wenzhou Municipal Science and Technology Bureau[H20080052]
WOS关键词	TANDEM MASS-SPECTROMETRY ; SOLVENT-EXTRACTION ; GAS-CHROMATOGRAPHY ; IN-VITRO ; GC-MS ; RATS ; MADECASSOSIDE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000268995600011
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25626]
专题	自然资源部第一海洋研究所
通讯作者	Han, Chao
作者单位	1.Wenzhou Entry Exit Inspect & Quarantine Bur PRC, Wenzhou 325027, Peoples R China 2.Wenzhou Univ, Coll Chem & Mat Sci, Wenzhou 325027, Peoples R China 3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol Engn, Hangzhou 310035, Zhejiang, Peoples R China 4.SOA, Inst Oceanog 1, Qingdao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Shen, Yan,Liu, Aili,Ye, Mingde,et al. Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS[J]. CHROMATOGRAPHIA,2009,70(3-4):431-438.
APA	Shen, Yan.,Liu, Aili.,Ye, Mingde.,Wang, Li.,Chen, Junhui.,...&Han, Chao.(2009).Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS.CHROMATOGRAPHIA,70(3-4),431-438.
MLA	Shen, Yan,et al."Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC-MS".CHROMATOGRAPHIA 70.3-4(2009):431-438.