Purification and characterization of cold-active endo-1,4-beta-glucanase produced by Pseudoalteromonas sp AN545 from Antarctica

doi:10.1007/s00343-011-0311-4

	Purification and characterization of cold-active endo-1,4-beta-glucanase produced by Pseudoalteromonas sp AN545 from Antarctica
	Shen Jihong 2; Kan Guangfeng 1; Shi Cuijuan 1; Lei Zhenhuan 1; Xie Qiuju 1; Qian Wenjia 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2011-09
卷号	29 期号:5 页码:1086-1092
关键词	Pseudoalteromonas endo-1,4-beta-glucanase cold-active enzyme Antarctic sea ice stability
ISSN号	0254-4059
DOI	10.1007/s00343-011-0311-4
英文摘要	A bacterium hydrolyzing carboxymethylcellulose, isolated from Antarctic sea ice, was identified as Pseudoalteromonas sp. based on 16S rDNA gene sequences and named as Pseudoalteromonas sp. AN545. The extracellular endo-1,4-beta-glucanase AN-1 was purified successively by ammonium sulfate precipitation, DEAE-Sepharose ion exchange chromatography and Sephadex G-75 gel filtration chromatography. The molecular mass of AN-1 was estimated to be 47.5 kDa utilizing SDS-PAGE and gel chromatography analysis. AN-1 could hydrolyze caboxymethylcellulose, avicel and beta-glucan, but not cellobiose, xylan and p-Nitrophenyl-beta-D-glucopyranoside. The optimal temperature and pH for the beta-glucanase activity of AN-1 were determined to be at 30A degrees C and pH 6.0, respectively. AN-1 was stable at acidic solutions of pH 5.0-6.5 and temperatures below 30A degrees C for 1 h. Moreover, the specific activity was enhanced by Ca(2+) and Mg(2+), and inhibited by Cu(2+). The kinetic parameters Michaelis constant (K (m)) and maximum velocity (V (max)) of AN-1 were 3.96 mg/mL and 6.06x10(-2) mg/(min center dot mL), respectively.
资助项目	Fundamental Research Funds for the Central Universities[HIT. IBRSEM.2009148]
WOS关键词	FIBROBACTER-SUCCINOGENES S85 ; CELLULASE ; BACILLUS ; ENDOGLUCANASE ; HALOPLANKTIS ; GLUCANASE ; PROTEIN
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000293632500022
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25205]
专题	自然资源部第一海洋研究所
通讯作者	Kan Guangfeng
作者单位	1.Harbin Inst Technol Weihai, Sch Ocean, Weihai 264209, Peoples R China 2.State Ocean Adm, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Shen Jihong,Kan Guangfeng,Shi Cuijuan,et al. Purification and characterization of cold-active endo-1,4-beta-glucanase produced by Pseudoalteromonas sp AN545 from Antarctica[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(5):1086-1092.
APA	Shen Jihong,Kan Guangfeng,Shi Cuijuan,Lei Zhenhuan,Xie Qiuju,&Qian Wenjia.(2011).Purification and characterization of cold-active endo-1,4-beta-glucanase produced by Pseudoalteromonas sp AN545 from Antarctica.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(5),1086-1092.
MLA	Shen Jihong,et al."Purification and characterization of cold-active endo-1,4-beta-glucanase produced by Pseudoalteromonas sp AN545 from Antarctica".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.5(2011):1086-1092.