Comparison of different buffer systems for separation of 15 nucleosides by capillary electrophoresis

doi:10.3724/SP.J.1123.2011.00481

	Comparison of different buffer systems for separation of 15 nucleosides by capillary electrophoresis
	Shi Qian 1; Chen Junhui 1; Li Xin 1; Cao Wei 1; Zheng Li 1; Zang Jiaye 1; Wang Xiaoru 1,2
刊名	CHINESE JOURNAL OF CHROMATOGRAPHY
	2011-06
卷号	29 期号:6 页码:481-487
关键词	capillary electrophoresis (CE) elctrospray ionization-time of flight mass spectromrtry (ESI-TOF/MS) nucleosides buffer systems
ISSN号	1000-8713
DOI	10.3724/SP.J.1123.2011.00481
英文摘要	The most suitable background electrolytes (BGEs) for simultaneous separation of 15 nucleosides by different modes of capillary electrophoresis (CE) were obtained. Various modes of CE were performed including capillary zone electrophoresis (CZE), capillary electrophoresis-electrospray ionization-time of flight mass spectrometry (CE-ESI-TOF/MS) and micellar electrokinetic chromatography (MEKC). The electrolyte buffers using sodium tetraborate decahydrate, disodium hydrogen phosphate, sodium acetate, sodium bicarbonate, ammonium acetate or 1, 2-diamino-ethane (DEA) were tested, and the best of them were systematically optimized. In CZE mode, the nucleosides could not be separated completely with sodium tetraborate decahydrate or disodium hydrogen phosphate as BGEs, demonstrating the limited applicability of the two buffer systems for complex samples. However, with 300 mmol/L DEA (containing 2% acetone) as BGE, 15 nucleosides could be separated with good resolution and peak shape, which proved that the DEA buffer was most suitable in CZE. The best buffer system in MEKC mode was 25 mmol/L disodium hydrogen phosphate with 70 mmol/L sodium dodecyl sulfate (SDS), and it was successfully applied for the separation of the nucleosides in Chinese Anthopleura lanthogrammica Berkly. The optimum buffer system for CE-ESI-TOF/MS analysis was 20 mmol/L ammonium acetate (pH 10.0). In the positive ion mode, the MS signals of each compound were better than those in the literature using DEA as BGE. The results of this study demonstrated the applicability of different buffer systems for the simultaneous separation of 15 nucleosides, and were helpful for the development of CE method in complex sample separation.
WOS研究方向	Chemistry
语种	中文
出版者	SCIENCE PRESS
WOS记录号	WOS:000435248800003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24730]
专题	自然资源部第一海洋研究所
通讯作者	Chen Junhui
作者单位	1.State Ocean Adm, Inst Oceanog 1, Qingdao Key Lab Analyt Technol & Standardizat Chi, Qingdao 266061, Peoples R China 2.Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Shi Qian,Chen Junhui,Li Xin,et al. Comparison of different buffer systems for separation of 15 nucleosides by capillary electrophoresis[J]. CHINESE JOURNAL OF CHROMATOGRAPHY,2011,29(6):481-487.
APA	Shi Qian.,Chen Junhui.,Li Xin.,Cao Wei.,Zheng Li.,...&Wang Xiaoru.(2011).Comparison of different buffer systems for separation of 15 nucleosides by capillary electrophoresis.CHINESE JOURNAL OF CHROMATOGRAPHY,29(6),481-487.
MLA	Shi Qian,et al."Comparison of different buffer systems for separation of 15 nucleosides by capillary electrophoresis".CHINESE JOURNAL OF CHROMATOGRAPHY 29.6(2011):481-487.