Promoting the treatment of crude oil alkane pollution through the study of enzyme activity

doi:10.1016/j.ijbiomac.2018.07.160

	Promoting the treatment of crude oil alkane pollution through the study of enzyme activity
	Meng, Long 1,2; Li, Wen 1,2; Bao, Mutai 1,2; Sun, Peiyan 3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-11
卷号	119 页码:708-716
关键词	Crude oil alkane bioremediation Pseudomonas Gene expression and enzyme activity
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.07.160
英文摘要	Microbes appear to play a key role in bioremediation of petroleum hydrocarbons pollution and little attention has been paid to the enzyme activity in the process of alkane bioremediation. Oil field bacterium identified as Pseudomonas synxantha LSH-7' was chosen as the tested strain. Periodically collected samples were analyzed by GC-FID (Gas Chromatography- Flame Ionization Detector) and RT-qPCR (Quantitative-Real-Time-PCR). GC-FID results showed this bacterial strain has great degradation ability on crude oil n-alkanes and RT-qPCR data indicated the differences between the three genes expression including AIkB-, Cytochromes P450-, and almA- related when grown on different-chain alkanes. Meanwhile, enzyme activity like alkane hydroxylase, alcohol dehydrogenase, dehydrogenase, protease, phosphatase, catalase and lipase were measured. Extracellular alkane hydroxylase was induced in a higher degree than intracellular in the early incubation time, alcohol dehydrogenase increased/decreased along with alkane hydroxylase, and the pH of the medium obviously decreased. Other enzymes were also described including dehydrogenase activity that reached a highest point that was slower than alcohol dehydrogenase, protease activity started multiplying after a period of culture while biomass was immediately increased, catalase activity dramatically enhanced in the presence of alkanes, phosphatase activity was closely linked to pH approximately but lipase activity was found to be moderate. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	Program for Innovative Research Team in University[IRT1289]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000447682100081
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/10821]
专题	自然资源部第一海洋研究所
通讯作者	Bao, Mutai
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 3.State Ocean Adm, North China Sea Environm Monitoring Ctr, Key Lab Marine Spill Oil Identificat & Damage Ass, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Meng, Long,Li, Wen,Bao, Mutai,et al. Promoting the treatment of crude oil alkane pollution through the study of enzyme activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,119:708-716.
APA	Meng, Long,Li, Wen,Bao, Mutai,&Sun, Peiyan.(2018).Promoting the treatment of crude oil alkane pollution through the study of enzyme activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,119,708-716.
MLA	Meng, Long,et al."Promoting the treatment of crude oil alkane pollution through the study of enzyme activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 119(2018):708-716.