Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy | |
Sun, Xiuqin; Zheng, Fengrong; Li, Jiye; Wu, Xing'an; Qu, Lingyun; Zhang, Jinxing; Hong, Xuguang | |
刊名 | Gaojishu Tongxin/Chinese High Technology Letters |
2006-07-01 | |
卷号 | 16期号:7页码:740-745 |
ISSN号 | 10020470 |
英文摘要 | Total RNA was extracted from a gill-derived cell strain FG-9307 of the Japanese flounder, and a fragment of major capsid protein encoding gene (approximately 0.6 kb in length) was amplified using RT-PCR and cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV0.6 kb. This plasmid successfully achieved immediate expression after being transferred by liposome into the eukaryotic cell strain FEC. pEGFP-N2-LCDV0.6 kb was injected into Paralichthys olivaceus intramuscularly and T-lymphopoiesis, respiratory burst in peripheral blood, hind-intestines, spleen, head-kidney and antibodies against LCDV were evaluated. The results indicated that the plasmid of pEGFP-N2-LCDV0.6 kb could induce a unique humoral and cell-mediated immune response and possess the function of immune protection. Therefore, further studies are necessary for the development and application of a DNA vaccine. |
语种 | 中文 |
出版者 | Inst. of Scientific and Technical Information of China, Beijing, China |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/6848] |
专题 | 自然资源部第一海洋研究所 |
作者单位 | 1.First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China; 2.Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China |
推荐引用方式 GB/T 7714 | Sun, Xiuqin,Zheng, Fengrong,Li, Jiye,et al. Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy[J]. Gaojishu Tongxin/Chinese High Technology Letters,2006,16(7):740-745. |
APA | Sun, Xiuqin.,Zheng, Fengrong.,Li, Jiye.,Wu, Xing'an.,Qu, Lingyun.,...&Hong, Xuguang.(2006).Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy.Gaojishu Tongxin/Chinese High Technology Letters,16(7),740-745. |
MLA | Sun, Xiuqin,et al."Construction of DNA vaccine against lymphocystis disease virus (LCDV) in flounders and evaluation of its immune efficacy".Gaojishu Tongxin/Chinese High Technology Letters 16.7(2006):740-745. |
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