The 25-kDa synaptosome-associated protein (SNAP-25) binds and inhibits delayed rectifier potassium channels in secretory cells.

doi:10.1074/jbc.M201034200

	The 25-kDa synaptosome-associated protein (SNAP-25) binds and inhibits delayed rectifier potassium channels in secretory cells.
	Ji, JZ ; Tsuk, S ; Salapatek, AMF ; Huang, XH ; Chikvashvili, D ; Pasyk, EA ; Kang, YH ; Sheu, L ; Tsushima, R ; Diamant, N
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2002-06-07
卷号	277 期号:23 页码:20195-20204
ISSN号	0021-9258
DOI	10.1074/jbc.M201034200
英文摘要	Delayed-rectilrier K+ channels (K-DR) are important regulators of membrane excitability in neurons and neuroendocrine cells. Opening of these voltage-dependent K+ channels results in membrane repolarization, leading to the closure of the Ca2+ channels and cessation of insulin secretion in neuroendocrine islet 13 cells. Using patch clamp techniques, we have demonstrated that the activity of the K-DR channel subtype, K(v)1.1, identified by its specific blocker dendrodotoxin-K, is inhibited by SNAP-25 in insulinoma HIT-T15 beta cells. A coprecipitation study of rat brain confirmed that SNAP-25 interacts with the K(v)1.1 protein. Cleavage of SNAP-25 by expression of botulinum neurotoxin A in HIT-T15 cells relieved this SNAP-25-mediated inhibition of KDR. This inhibitory effect of SNAP-25 is mediated by the N terminus of K(v)1.1, likely by direct interactions with K(v)1.1 and/or K(v)beta subunits, as revealed by co-immunoprecipitation performed in the Xenopus oocyte expression system and in vitro binding. Taken together we have concluded that SNAP-25 mediates secretion not only through its participation in the exocytotic SNARE complex but also by regulating membrane potential and calcium entry through its interaction with K-DR channels.
URL标识	查看原文
资助项目	Canadian Institutes of Health Research[42394]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000176204500015
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4873]
专题	自然资源部第一海洋研究所
作者单位	1.Univ Toronto, Dept Med, Toronto, ON M5S 1A8, Canada; 2.Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada; 3.Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada; 4.Hosp Sick Children, Cell Biol Program, Toronto, ON M5G 1X8, Canada; 5.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China; 6.Tel Aviv Univ, Sackler Sch Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
推荐引用方式 GB/T 7714	Ji, JZ,Tsuk, S,Salapatek, AMF,et al. The 25-kDa synaptosome-associated protein (SNAP-25) binds and inhibits delayed rectifier potassium channels in secretory cells.[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2002,277(23):20195-20204.
APA	Ji, JZ.,Tsuk, S.,Salapatek, AMF.,Huang, XH.,Chikvashvili, D.,...&Gaisano, HY.(2002).The 25-kDa synaptosome-associated protein (SNAP-25) binds and inhibits delayed rectifier potassium channels in secretory cells..JOURNAL OF BIOLOGICAL CHEMISTRY,277(23),20195-20204.
MLA	Ji, JZ,et al."The 25-kDa synaptosome-associated protein (SNAP-25) binds and inhibits delayed rectifier potassium channels in secretory cells.".JOURNAL OF BIOLOGICAL CHEMISTRY 277.23(2002):20195-20204.