Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida : Stichopodidae) and cloning of ependymin gene

doi:10.1007/s10750-006-0231-z

	Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida : Stichopodidae) and cloning of ependymin gene
	Zheng, Fa-Xin ; Sun, Xiu-Qin ; Fang, Bao-Hai ; Hong, Xu-Guang ; Zhang, Jin-Xing
刊名	HYDROBIOLOGIA
	2006-11
卷号	571 页码:109-122
关键词	Apostichopus japonicus ependymin encoding gene expressed sequence tag regenerating intestine regeneration associated gene
ISSN号	0018-8158
DOI	10.1007/s10750-006-0231-z
英文摘要	The regeneration of the intestine of sea cucumber (Apostichopus japonicus) was studied by describing historically the changes that occurred during intestine regeneration on the fifth day after chemically-induced evisceration. An expressed sequence tag (EST) analysis was undertaken to identify major genes, which might be involved in intestine regeneration of A. japonicus. Two cDNA libraries were constructed with directional cloning method, one for regenerating intestine collected on the third, fourth and fifth day after evisceration (post-evisceration, PE), and the other for the non-eviscerated (NE). A total of 730 ESTs were generated by sequencing cDNA clones from the two libraries (372 from PE and 358 from NE). The results showed that the number of genes that were involved in primary metabolism of PE library was less than that of NE library, while the number of genes involved in cell defense/immunity, cell division, cell signal transduction/communication of PE library was more than that of NE library. The results also revealed that the expression of the genes which might be involved in regeneration was enhanced to some extent after evisceration. Only about 11.54% of the sequenced clones were shared by two libraries, which provided some clues for the existence of differential gene expression between PE and NE intestines. A gene named epenAj was also characterized in this study.
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000240308500008
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4632]
专题	自然资源部第一海洋研究所
作者单位	1.SOA, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China; 2.Ocean Univ China, Qingdao 266003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fa-Xin,Sun, Xiu-Qin,Fang, Bao-Hai,et al. Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida : Stichopodidae) and cloning of ependymin gene[J]. HYDROBIOLOGIA,2006,571:109-122.
APA	Zheng, Fa-Xin,Sun, Xiu-Qin,Fang, Bao-Hai,Hong, Xu-Guang,&Zhang, Jin-Xing.(2006).Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida : Stichopodidae) and cloning of ependymin gene.HYDROBIOLOGIA,571,109-122.
MLA	Zheng, Fa-Xin,et al."Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida : Stichopodidae) and cloning of ependymin gene".HYDROBIOLOGIA 571(2006):109-122.