Chitosan microspheres as candidate plasmid vaccine carrier for oral immunisation of Japanese flounder (Paralichthys olivaceus)

doi:10.1016/j.vetimm.2008.07.002

	Chitosan microspheres as candidate plasmid vaccine carrier for oral immunisation of Japanese flounder (Paralichthys olivaceus)
	Tian, Jiyuan 2; Yu, Juan 1; Sun, Xiuqin 3
刊名	VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
	2008-12-15
卷号	126 期号:3-4 页码:220-229
关键词	Chitosan microspheres Drug delivery DNA vaccine Protein expression
ISSN号	0165-2427
DOI	10.1016/j.vetimm.2008.07.002
英文摘要	Oral DNA-based immunotherapy is a new treatment option for fish immunisation in intensive culture. However, because of the existence of the nucleases and severe gastrointestinal conditions, DNA-based vaccines can be hydrolyzed or denatured. In our laboratory, a plasmid DNA (pDNA) containing major capsid protein (MCP) gene of lymphocystis disease virus (LCDV) was prepared, and then pDNA was encapsulated in chitosan microspheres through an emulsion-based methodology. The yield, loading percent and encapsulation efficiency of microspheres were 93.6%, 0.3% and 94.5%, respectively. Scanning electron microscopy (SEM) showed that pDNA-loaded microspheres yielded a spherical shape with smooth surfaces. The disproportion of super-coiled to open circle and linear pDNA suggested that high transfection efficiencies of pDNA in microspheres were retained. The cumulative release of pDNA showed that chitosan microspheres were resistant to degradation in simulated gastrointestinal tract environment. The release profile at PBS buffer (pH 7.4) displayed that pDNA-loaded chitosan microspheres had a release up to 42 days after intestinal imbibition. RT-PCR showed that RNA containing information of MCP gene existed in various tissues 10-90 days post-vaccination. SDS-PAGE and immunofluorescent images indicated that pDNA expressed MCP in tissues of fish 10-90 days after oral administration. In addition, indirect ELISA displayed that the immune responses of sera were positive (O.D. >= 0.3) from week I to week 16 for fish vaccinated with microspheres, in comparison with fish vaccinated with naked pDNA. Data obtained suggested that chitosan microspheres were promising carriers for oral pDNA vaccine. Because this encapsulation technique was easy to operate and immunisation efficacy of microspheres loaded with pDNA was significant, it had potential to be used in drug delivery applications. (C) 2008 Elsevier B.V. All rights reserved.
资助项目	National High Technology Research and Development Program of China[2003AA622030]
WOS研究方向	Immunology ; Veterinary Sciences
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000261867500004
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4450]
专题	自然资源部第一海洋研究所
作者单位	1.Ocean Univ China, Coll Chem & Chem Engn, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China; 2.Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao 266109, Peoples R China; 3.State Oceanog Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Tian, Jiyuan,Yu, Juan,Sun, Xiuqin. Chitosan microspheres as candidate plasmid vaccine carrier for oral immunisation of Japanese flounder (Paralichthys olivaceus)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2008,126(3-4):220-229.
APA	Tian, Jiyuan,Yu, Juan,&Sun, Xiuqin.(2008).Chitosan microspheres as candidate plasmid vaccine carrier for oral immunisation of Japanese flounder (Paralichthys olivaceus).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,126(3-4),220-229.
MLA	Tian, Jiyuan,et al."Chitosan microspheres as candidate plasmid vaccine carrier for oral immunisation of Japanese flounder (Paralichthys olivaceus)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 126.3-4(2008):220-229.