Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination | |
Zheng, Feng Rong1; Sun, Xiu Qin1; Xing, Ming Qing1; Liu, Hongzhan2 | |
刊名 | AQUACULTURE RESEARCH
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2010-09-16 | |
卷号 | 41期号:10页码:1444-1451 |
关键词 | Japanese flounder lymphocystis disease virus DNA vaccine major histocompatibility class TCR immune-related gene |
ISSN号 | 1355-557X |
DOI | 10.1111/j.1365-2109.2009.02435.x |
英文摘要 | In this study, we found that an intramuscular injection of Japanese flounder (Paralichthys olivaceus, 60-80 g in weight and 15-20 mL in length) with 5 mu g of a DNA vaccine (pEGFP-N2-LCDV-cn-MCP 0.6 kb, containing lymphocystis disease virus major capsid protein gene) induced a strong immune response. Subsequent real-time polymerase chain reaction showed that the expression of immune-related genes [e.g., major histocompatibility complex (MHC) class I alpha, MHC II alpha, T-cell receptor (TCR), tumour necrosis factor (TNF), tumour necrosis factor receptor (TNFR), Mx, interleukin (IL)-1 beta, CXC and IL-8R] was significantly changed after DNA vaccination. The most remarkable alternation was the expression of MHC I alpha and MHC II alpha genes: MHC II alpha reached the maximum on day 8 in different tissues, and MHC I alpha on day 2 in the intestine and gills. The expression of TCR increased and reached a plateau in 2 days in the spleen, gills, kidney and liver after vaccination and then decreased after day 8. In contrast, the expression of TCR in the intestine increased and reached a plateau in 8 days. The expression of IL-8R reached the maximum on day 2 in different tissues and then decreased on day 8. Mx increased in the gills, kidney, spleen and liver on days 2, 8, 2 and 2, but decreased in the intestine, gills, spleen and liver on days 2, 8, 8 and 8 respectively. The TNFR expression increased in the spleen, kidney and gills on days 2, 8 and 8, but decreased in intestine, liver and gills on days 2, 8 and 8 respectively. The expression of TNF, CXC and IL-1 beta increased 2 and 8 days after the injection of DNA vaccine. However, the expression of TNF, CXC and IL-1 beta altered on days 2 and 8 with different patterns in different tissues respectively. The fish responded to the DNA vaccine by yielding a specific immunoglobulin against lymphocystis disease virus (LCDV) as observed with indirect ELISA. The DNA vaccine induced a unique humoral response, suggesting that the DNA vaccine activated both cellular and humoral defences of the specific immune system of Japanese flounder. |
资助项目 | National High-tech Research and Development (863) Program of China[2003AA622030] ; National High-tech Research and Development (863) Program of China[2006AA100309] |
WOS研究方向 | Fisheries |
语种 | 英语 |
出版者 | WILEY-BLACKWELL PUBLISHING, INC |
WOS记录号 | WOS:000282178000044 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn/handle/2SI8HI0U/4296] ![]() |
专题 | 自然资源部第一海洋研究所 |
作者单位 | 1.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Shandong Univ, Marine Coll, Weihai, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Feng Rong,Sun, Xiu Qin,Xing, Ming Qing,et al. Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination[J]. AQUACULTURE RESEARCH,2010,41(10):1444-1451. |
APA | Zheng, Feng Rong,Sun, Xiu Qin,Xing, Ming Qing,&Liu, Hongzhan.(2010).Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination.AQUACULTURE RESEARCH,41(10),1444-1451. |
MLA | Zheng, Feng Rong,et al."Immune response of DNA vaccine against lymphocystis disease virus and expression analysis of immune-related genes after vaccination".AQUACULTURE RESEARCH 41.10(2010):1444-1451. |
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