Efficient sample proteolysis based on a microchip containing a glass fiber core with immobilized trypsin

doi:10.1007/s00604-012-0896-0

	Efficient sample proteolysis based on a microchip containing a glass fiber core with immobilized trypsin
	Bao, Huimin 1; Liu, Shuang 2; Zhang, Luyan 1; Chen, Gang 1
刊名	MICROCHIMICA ACTA
	2012-11
卷号	179 期号:3-4 页码:291-297
关键词	Microchip Trypsin Immobilization Oxidized cellulose Proteolysis
ISSN号	0026-3672
DOI	10.1007/s00604-012-0896-0
英文摘要	Trypsin was immobilized on cellulose-coated glass fibers via a condensation reaction between the aldehyde groups of the oxidized cellulose and the primary amino groups of trypsin. A piece of the modified fiber was inserted into the main channel of a poly(methyl methacrylate) microchip to form a microfluidic proteolytic bioreactor. Scanning electron microscopy of the cross section of the fiber revealed a rough film on the surface of the fiber glass. The performance of the bioreactor was demonstrated by the tryptic digestion of hemoglobin and cytochrome c, where the time for digestion was reduced to < 10 s. The digests were identified by MALDI-TOF-MS to obtain peptide mass fingerprint spectra. The results indicated that the digestion in the microfluidic bioreactor is comparable to that of a 12-h solution tryptic digest and thus provides a promising platform for the high throughput identification of proteins.
资助项目	Education Ministry of China[NCET-08-0134]
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000310222500014
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4054]
专题	自然资源部第一海洋研究所
作者单位	1.Fudan Univ, Sch Pharm, Dept Chem, Shanghai 200032, Peoples R China; 2.State Ocean Adm, N China Sea Environm Monitoring Ctr, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Bao, Huimin,Liu, Shuang,Zhang, Luyan,et al. Efficient sample proteolysis based on a microchip containing a glass fiber core with immobilized trypsin[J]. MICROCHIMICA ACTA,2012,179(3-4):291-297.
APA	Bao, Huimin,Liu, Shuang,Zhang, Luyan,&Chen, Gang.(2012).Efficient sample proteolysis based on a microchip containing a glass fiber core with immobilized trypsin.MICROCHIMICA ACTA,179(3-4),291-297.
MLA	Bao, Huimin,et al."Efficient sample proteolysis based on a microchip containing a glass fiber core with immobilized trypsin".MICROCHIMICA ACTA 179.3-4(2012):291-297.