A Population-Size Model for Protein Spot Detection in Proteomic Studies | |
Mao, Chang Xuan1; Chen, Sining2; Yang, Yitong1 | |
刊名 | JOURNAL OF AGRICULTURAL BIOLOGICAL AND ENVIRONMENTAL STATISTICS
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2016-03 | |
卷号 | 21期号:1页码:170-180 |
关键词 | Binomial mixture Capture-recapture Lower bounds Nonidentifiability |
ISSN号 | 1085-7117 |
DOI | 10.1007/s13253-015-0221-6 |
英文摘要 | In proteomic studies, a population of proteins are often examined on a gel using a technique called two-dimensional gel eletrophoresis. The technique separates the protein population into individual protein spots on a two-dimensional gel by isoelectric charge and molecular weight. The resulting gel images are then processed by a software system for spot detection and subsequent analysis. The performance of a spot-detection program is evaluated by the total number of spots that are detected. A popular spot-detection program uses the "master-slave" approach, where all spots on "slave images" are subsets of the spots on the "master image." We argue that this approach potentially misses a large proportion of proteins and propose a model that quantifies the lack of performance. We provide nonparametric estimators for the protein population size and the expected number of proteins to be detected if a "fusion-gel" approach was used. Using the data from a rat liver proteome study, we estimate that more than half of the protein population is missed by the master-slave approach. |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Mathematical & Computational Biology ; Mathematics |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000374695400010 |
内容类型 | 期刊论文 |
源URL | [http://10.2.47.112/handle/2XS4QKH4/1330] ![]() |
专题 | 上海财经大学 |
通讯作者 | Mao, Chang Xuan |
作者单位 | 1.Shanghai Univ Finance & Econ, Sch Stat & Management, Shanghai 200433, Peoples R China; 2.Bell Labs, Dept Stat & Learning, 600 Mt Ave, Murray Hill, NJ 07974 USA |
推荐引用方式 GB/T 7714 | Mao, Chang Xuan,Chen, Sining,Yang, Yitong. A Population-Size Model for Protein Spot Detection in Proteomic Studies[J]. JOURNAL OF AGRICULTURAL BIOLOGICAL AND ENVIRONMENTAL STATISTICS,2016,21(1):170-180. |
APA | Mao, Chang Xuan,Chen, Sining,&Yang, Yitong.(2016).A Population-Size Model for Protein Spot Detection in Proteomic Studies.JOURNAL OF AGRICULTURAL BIOLOGICAL AND ENVIRONMENTAL STATISTICS,21(1),170-180. |
MLA | Mao, Chang Xuan,et al."A Population-Size Model for Protein Spot Detection in Proteomic Studies".JOURNAL OF AGRICULTURAL BIOLOGICAL AND ENVIRONMENTAL STATISTICS 21.1(2016):170-180. |
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