From Solution to Biointerface: Graphene Self-Assemblies of Varying   Lateral Sizes and Surface Properties for Biofilm Control and   Osteodifferentiation

doi:10.1021/acsami.6b05198

CORC > 北京大学 > 工学院

	From Solution to Biointerface: Graphene Self-Assemblies of Varying Lateral Sizes and Surface Properties for Biofilm Control and Osteodifferentiation
	Jia, Zhaojun ; Shi, Yuyin ; Xiong, Pan ; Zhou, Wenhao ; Cheng, Yan ; Zheng, Yufeng ; Xi, Tingfei ; Wei, Shicheng
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016
关键词	self-assembly poly(dopamine) graphene nanomaterials antibacterial cytotoxicity osteogenic MUSSEL-INSPIRED POLYDOPAMINE BONE MORPHOGENIC PROTEIN-2 BACTERIAL ADHESION ANTIBACTERIAL ACTIVITY OSTEOBLAST FUNCTIONS OXIDE NANOSHEETS STEM-CELLS FUNCTIONALIZATION MECHANISMS TITANIUM
DOI	10.1021/acsami.6b05198
英文摘要	Bringing multifunctional graphene out of solution through facile self assembly to form 2D surface nanostructures, with control over the lateral size and surface properties, would be an intriguing accomplishment, especially in biomedical fields where biointerfaces with functional diversity are in high demand. Guided by this goal, in this work, we built such graphene-based self-assemblies on orthopedic titanium, attempting to selectively regulate bacterial activities and osteoblastic functions, which are both crucial in bone regeneration. Briefly, large-area graphene oxide (GO) sheets and functionalized reduced GO (rGO) micro-/nanosheets were self-assembled spontaneously and controllably onto solid Ti, through an evaporation-assisted electrostatic assembly process and a mussel inspired one-pot assembly process, respectively. The resultant layers were characterized in terms of topological structure, chemical composition, hydrophilicity, and protein adsorption properties. The antibacterial efficacies of the assemblies were examined by challenging them with pathogenic Staphylococcus aureus (S. aureus) bacteria that produce biofilms, whereby around 50% antiadhesion effects and considerable antibiofilm activities were observed for both layer types but through dissimilar modes of action. Their cytocompatibility and osteogenic potential were also investigated. Interfaced with MC3T3-E1 cells, the functionalized rGO sheets evoked better cell adhesion and growth than GO sheets, whereas the latter elicited higher osteodifferentiation activity throughout a 28-day in vitro culture. In this work, we showed that it is technically possible to construct graphene interface layers of varying lateral dimensions and surface properties and confirmed the concept of using the obtained assemblies to address the two major challenges facing orthopedic clinics. In addition, we determined fundamental implications for understanding the surface biology relationship of graphene biomaterials, in efforts to better design and more safely use them for future biomedicine.; National Natural Science Foundation of China [31370954, 51431002]; Project of Scientific and Technical Plan of Beijing [Z141100002814008]; State Key Laboratory of Bioelectronics Open Research Fund of China (Chien-Shiung Wu Laboratory) [08400-413-161-002]; China Scholarship Council (CSC) [201506010201]; SCI(E); EI; PubMed; ARTICLE; chengyan@pku.edu.cn; 27; 17151-17165; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/491893]
专题	工学院
推荐引用方式 GB/T 7714	Jia, Zhaojun,Shi, Yuyin,Xiong, Pan,et al. From Solution to Biointerface: Graphene Self-Assemblies of Varying Lateral Sizes and Surface Properties for Biofilm Control and Osteodifferentiation[J]. ACS APPLIED MATERIALS & INTERFACES,2016.
APA	Jia, Zhaojun.,Shi, Yuyin.,Xiong, Pan.,Zhou, Wenhao.,Cheng, Yan.,...&Wei, Shicheng.(2016).From Solution to Biointerface: Graphene Self-Assemblies of Varying Lateral Sizes and Surface Properties for Biofilm Control and Osteodifferentiation.ACS APPLIED MATERIALS & INTERFACES.
MLA	Jia, Zhaojun,et al."From Solution to Biointerface: Graphene Self-Assemblies of Varying Lateral Sizes and Surface Properties for Biofilm Control and Osteodifferentiation".ACS APPLIED MATERIALS & INTERFACES (2016).