Super-resolution dipole orientation mapping via polarization   demodulation

doi:10.1038/lsa.2016.166

CORC > 北京大学 > 工学院

	Super-resolution dipole orientation mapping via polarization demodulation
	Zhanghao, Karl ; Chen, Long ; Yang, Xu-San ; Wang, Miao-Yan ; Jing, Zhen-Li ; Han, Hong-Bin ; Zhang, Michael Q. ; Jin, Dayong ; Gao, Jun-Tao ; Xi, Peng
刊名	LIGHT-SCIENCE & APPLICATIONS
	2016
关键词	dipole fluorescence polarization microscopy orientation mapping polarization modulation super-resolution MEASURING ROTATIONAL DIFFUSION FLUORESCENCE MICROSCOPY LOCALIZATION MICROSCOPY ADDITIONAL INFORMATION MODULATION ADDS ORGANIZATION MEMBRANES CELLS ORDER YEAST
DOI	10.1038/lsa.2016.166
英文摘要	Fluorescence polarization microscopy (FPM) aims to detect the dipole orientation of fluorophores and to resolve structural information for labeled organelles via wide-field or confocal microscopy. Conventional FPM often suffers from the presence of a large number of molecules within the diffraction-limited volume, with averaged fluorescence polarization collected from a group of dipoles with different orientations. Here, we apply sparse deconvolution and least-squares estimation to fluorescence polarization modulation data and demonstrate a super-resolution dipole orientation mapping (SDOM) method that resolves the effective dipole orientation from a much smaller number of fluorescent molecules within a sub-diffraction focal area. We further apply this method to resolve structural details in both fixed and live cells. For the first time, we show that different borders of a dendritic spine neck exhibit a heterogeneous distribution of dipole orientation. Furthermore, we illustrate that the dipole is always perpendicular to the direction of actin filaments in mammalian kidney cells and radially distributed in the hourglass structure of the septin protein under specific labelling. The accuracy of the dipole orientation can be further mapped using the orientation uniform factor, which shows the superiority of SDOM compared with its wide-field counterpart as the number of molecules is decreased within the smaller focal area. Using the inherent feature of the orientation dipole, the SDOM technique, with its fast imaging speed (at sub-second scale), can be applied to a broad range of fluorescently labeled biological systems to simultaneously resolve the valuable dipole orientation information with super-resolution imaging.; National Key Basic Research Program (973 Program) [2012CB316503]; National Instrument Development Special Program [2013YQ03065102]; National Natural Science Foundation of China [31361163004, 31327901, 61475010, 61428501]; UTD funds; SCI(E); 中国科技核心期刊(ISTIC); ARTICLE; dayong.jin@uts.edu.au; jtgao@biomed.tsinghua.edu.cn; xipeng@pku.edu.cn; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/458819]
专题	工学院
推荐引用方式 GB/T 7714	Zhanghao, Karl,Chen, Long,Yang, Xu-San,et al. Super-resolution dipole orientation mapping via polarization demodulation[J]. LIGHT-SCIENCE & APPLICATIONS,2016.
APA	Zhanghao, Karl.,Chen, Long.,Yang, Xu-San.,Wang, Miao-Yan.,Jing, Zhen-Li.,...&Xi, Peng.(2016).Super-resolution dipole orientation mapping via polarization demodulation.LIGHT-SCIENCE & APPLICATIONS.
MLA	Zhanghao, Karl,et al."Super-resolution dipole orientation mapping via polarization demodulation".LIGHT-SCIENCE & APPLICATIONS (2016).