Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with   Demineralized Bone Matrix in Chitosan-alginate Beads

doi:10.5405/jmbe.1137

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	Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads
	Sutradhar, Bibek Chandra ; Hong, Gyeongmi ; Ge, Zigang ; Kim, Gonhyung
刊名	journal of medical and biological engineering
	2013
关键词	Co-culture Chondrocytes Chitosan Alginate Beads MESENCHYMAL STEM-CELLS IN-VITRO ARTICULAR-CARTILAGE STROMAL CELLS TISSUE SCAFFOLDS HYDROGELS HYDROXYAPATITE BIOMATERIAL DERIVATIVES
DOI	10.5405/jmbe.1137
英文摘要	Cartilage injury leads to osteoarthritis and subsequent loss of mobility irreversibly if untreated. There is no widely established technology for the functional repair of large osteochondral defects, though some progress has been made in repairing/regenerating cartilage defects. In the present study, bovine chondrocytes were cultured in alginate or chitosan-alginate beads with and without co-culture of demineralized bone matrix (DBM) in vitro. After the four systems were maintained in vitro for four weeks, the cell morphology, proliferation, sulfated glycosaminoglycan (sGAG) synthesis, and gene expression were evaluated. The typical chondrocytic phenotype was maintained in all beads for the entire four weeks. The proliferation of chondrocytes in chitosan-alginate beads co-cultured with DBM was significantly (p < 0.05) higher than that in alginates. Histological analysis showed the typical round, plump morphology associated with an increased number of lacunae in chitosan-alginate beads. The production of sGAG also significantly (p < 0.05) increased in quality and quantity and type II collagen gene expression was maintained in the co-culture in chitosan-alginate beads. These results indicate that chondrocytes co-cultured with DBM in chitosan-alginate beads provide a preferred environment for high-density chondrogenesis with matrix synthesis, which may be considered as a sophisticated scaffold model for osteochondral tissue regeneration.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000327477900013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; SCI(E); EI; 1; ARTICLE; 5; 518-525; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392207]
专题	工学院
推荐引用方式 GB/T 7714	Sutradhar, Bibek Chandra,Hong, Gyeongmi,Ge, Zigang,et al. Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads[J]. journal of medical and biological engineering,2013.
APA	Sutradhar, Bibek Chandra,Hong, Gyeongmi,Ge, Zigang,&Kim, Gonhyung.(2013).Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads.journal of medical and biological engineering.
MLA	Sutradhar, Bibek Chandra,et al."Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads".journal of medical and biological engineering (2013).