An in vitro metabolic system of gut flora and the metabolism of   ginsenoside Rg3 and cholic acid

doi:10.1007/s13318-013-0143-z

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	An in vitro metabolic system of gut flora and the metabolism of ginsenoside Rg3 and cholic acid
	Zhao, Chunyan ; Sun, Runbin ; Cao, Bei ; Gu, Shenghua ; Zhao, Jieyu ; Liu, Linsheng ; Wang, Xinwen ; Zha, Weibin ; Yu, Xiaoyi ; Xiao, Wenjing ; Mao, Yong ; Ge, Chun ; Ju, Jiaqi ; Aa, Lixiang ; Fei, Fei ; Ding, Yi ; Aa, Jiye ; Wang, Guangji
刊名	european journal of drug metabolism and pharmacokinetics
	2014
关键词	In vitro Gut flora Metabolism Ginsenoside Rg3 Cholic acid HUMAN INTESTINAL BACTERIA BIOLOGICAL-ACTIVITIES SAPONINS MICROFLORA ABSORPTION TRACT DRUGS CELLS
DOI	10.1007/s13318-013-0143-z
英文摘要	For orally administered drugs, the metabolism of a drug by the gut flora plays an important role in the bioavailability, activation and disposition of the drug in vivo. However, no in vitro system is currently available to evaluate the metabolism of a drug by the gut flora before the drug is absorbed into the body. This paper presents an in vitro metabolic system in an anaerobic environment that could be used to evaluate the metabolism of an endogenous compound, cholic acid, and a xenobiotic compound, ginsenoside Rg3. We showed that the proliferation of the anaerobic bacteria of the gut content of hamsters produced a similar composition of gut flora in a culture medium for yeast to that in vivo. Incubation of ginsenoside Rg3 and cholic acid in the anaerobic in vitro system efficiently produced the metabolites Rh2 and deoxycholic acid, respectively, similar to those seen in the gut content in vivo. In comparison with in vivo analysis, this anaerobic in vitro metabolic system is convenient, reproducible, economic and animal saving, and can easily be applied to assess the transformation and disposition of a drug before it enters into the circulatory system.; Pharmacology & Pharmacy; SCI(E); 0; ARTICLE; jiye_2005@yahoo.com.cn; guangjiwang@hotmail.com; 2; 129-137; 39
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389294]
专题	工学院
推荐引用方式 GB/T 7714	Zhao, Chunyan,Sun, Runbin,Cao, Bei,et al. An in vitro metabolic system of gut flora and the metabolism of ginsenoside Rg3 and cholic acid[J]. european journal of drug metabolism and pharmacokinetics,2014.
APA	Zhao, Chunyan.,Sun, Runbin.,Cao, Bei.,Gu, Shenghua.,Zhao, Jieyu.,...&Wang, Guangji.(2014).An in vitro metabolic system of gut flora and the metabolism of ginsenoside Rg3 and cholic acid.european journal of drug metabolism and pharmacokinetics.
MLA	Zhao, Chunyan,et al."An in vitro metabolic system of gut flora and the metabolism of ginsenoside Rg3 and cholic acid".european journal of drug metabolism and pharmacokinetics (2014).