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Analytical description of high-aperture STED resolution with 0-2 pi vortex phase modulation
Xie, Hao ; Liu, Yujia ; Jin, Dayong ; Santangelo, Philip J. ; Xi, Peng
刊名journal of the optical society of america a optics image science and vision
2013
关键词ELECTROMAGNETIC DIFFRACTION FLUORESCENCE MICROSCOPY STIMULATED-EMISSION OPTICAL SYSTEMS IMAGE FIELD DEPLETION PERFORMANCE PATTERNS CENTERS LIGHT
DOI10.1364/JOSAA.30.001640
英文摘要Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0-2 pi vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. (C) 2013 Optical Society of America; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322591800022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Optics; SCI(E); EI; 6; ARTICLE; 8; 1640-1645; 30
语种英语
内容类型期刊论文
源URL[http://ir.pku.edu.cn/handle/20.500.11897/315867]  
专题工学院
推荐引用方式
GB/T 7714
Xie, Hao,Liu, Yujia,Jin, Dayong,et al. Analytical description of high-aperture STED resolution with 0-2 pi vortex phase modulation[J]. journal of the optical society of america a optics image science and vision,2013.
APA Xie, Hao,Liu, Yujia,Jin, Dayong,Santangelo, Philip J.,&Xi, Peng.(2013).Analytical description of high-aperture STED resolution with 0-2 pi vortex phase modulation.journal of the optical society of america a optics image science and vision.
MLA Xie, Hao,et al."Analytical description of high-aperture STED resolution with 0-2 pi vortex phase modulation".journal of the optical society of america a optics image science and vision (2013).
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